PXD062611-1

PXD062611 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Co-IP interactome studies of FRS2/B7-H3 ± C7/Chloroquine in UW228 cells
Description	Fibroblast growth factor receptor substrate 2 (FRS2) and B7-H3/CD276 are both highly expressed in medulloblastoma (MB) patient samples. While FRS2 has been shown to drive an invasive tumor phenotype, the biological role of B7-H3/CD276 is still incompeletely understood. Here, we explored the interactome of FRS2 and B7-H3/CD276 in MB cells stimulated with bFGF. Further, we studied the treatment impact with the FRS2 small molecule ligand C7 on both interactomes, as well as the effect of chloroquine on the interactome of B7-H3/CD276. Towards this aim, we expressed mNeonGreen-tagged FRS2 and B7-H3/CD276 in UW228 cells and isolated direct interactor proteins via pulldown using mNeonGreen-trap magnetic agarose beads. We performed mass spectrometry and quantified relative abundances compared to pulldowns from wild type UW228 cells.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:19:23.962.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Baumgartner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-04 08:15:30	ID requested
⏵ 1	2026-09-06 16:19:24	announced

Publication List

Kopp LL, Versamento D, Ciraulo B, Sch, ö, nholzer MT, Hochuli D, Lin MS, Baumgartner M, Fibroblast growth factor receptor substrate 2 interactome mapping reveals novel candidate interactors associated with migration and invasion. Cell Commun Signal, 24(1):(2026) [pubmed]
10.1186/s12964-026-02943-8;

Kopp LL, Versamento D, Ciraulo B, Sch, ö, nholzer MT, Hochuli D, Lin MS, Baumgartner M, Fibroblast growth factor receptor substrate 2 interactome mapping reveals novel candidate interactors associated with migration and invasion. Cell Commun Signal, 24(1):(2026) [pubmed]

10.1186/s12964-026-02943-8;

Keyword List

submitter keyword: pediatric, Chloroquine,Co-immunoprecipitation, Medulloblastoma, C7, B7-H3, UW228, CD276, brain cancer, FRS2

submitter keyword: pediatric, Chloroquine,Co-immunoprecipitation, Medulloblastoma, C7, B7-H3, UW228, CD276, brain cancer, FRS2

Contact List

Martin Baumgartner
contact affiliation	University Children's Hospital Zürich
contact email	martin.baumgartner@kispi.uzh.ch
lab head
Martin Baumgartner
contact affiliation	University Children's Hospital Zürich
contact email	martin.baumgartner@kispi.uzh.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD062611
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD062611

PRIDE project URI

Repository Record List

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