PXD062608-1

PXD062608 is an original dataset announced via ProteomeXchange.

Title	Proteome of liver, intestine and muscle of weaning piglets in response to dietary inclusion of Chlorella vulgaris or its combination with Enterococcus lactis probiotic
Description	Maize and soybean meal are widely used in monogastric animal diets. However, their production raises sustainability concerns. Microalgae, like Chlorella vulgaris, are a potential alternative feedstuff due to their interesting nutritional profile, particularly a high crude protein content. As part of a study investigating the individual and combined effects of dietary C. vulgaris and probiotic Enterococcus lactis SF68 on weaning piglet growth performance, dietary utilization, digestibility, organ morphometrics and physiology by metabolomics, lipidomics and proteomics, the submitted dataset includes the proteomics approach. At weaning (28 days old), Piétrain x (Landrace x Large White) piglets were obtained from a commercial farm. Animals were housed in groups of three in 2m2 pens and, for five weeks, fed one of three diets ad libitum: a control diet based on cereals and soybean meal (Control); a diet with 5% C. vulgaris (CV5); and a diet with 5% C. vulgaris and E. lactis SF68 probiotic (CV5P). Liver, ileum, and muscle tissues were sampled and proteomic analysis performed by LC-MS/MS. Proteomics analysis identified 1966 proteins in the liver, 1592 in the intestine and 1020 in the muscle. In general, multivariate analyses were able to identify broad proteome differences in the liver, but not in the intestine nor muscle. Nonetheless, all organs exhibited notable changes in levels of individual proteins by univariate analyses. Analyses of differential abundance revealed that the probiotic was a major driver of the observed effects whereas the combination of C. vulgaris and probiotic resulted in fewer differentially abundant proteins than expected from an additive effect of individual factors. This suggests the existence of complex interactions between the two dietary components. Overall, results favor the combination of C. vulgaris with the probiotic. In the liver, the combined diet effect resulted in immune attenuation as well as maintained protein catabolism, attenuated mobilization of lipid reserves, and modulation of cellular activity. These effects could enhance the systemic availability of amino acids and lipids to support muscle development during the post-weaning growth stage. In the intestine, evidence of lower ketone oxidation suggests an improved nutritional state, along with evidence of immune priming against potential bacterial infections in piglets fed the CV5P diet. In the muscle, the combined diet effect drives changes in muscle fiber types by affecting connective tissue, mitochondrial respiration and regulation of lipid composition. These findings suggest a positive outcome in expected meat quality, potentially improving tenderness and nutritional value. The output of this dataset discloses the importance of considering the intricate interplay between dietary components and their combined effects on multi-organ systems.
HostingRepository	PRIDE
AnnounceDate	2026-02-10
AnnouncementXML	Submission_2026-02-10_13:08:39.780.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-04 08:09:47	ID requested
⏵ 1	2026-02-10 13:08:40	announced

10.1021/ACSAGSCITECH.5C00644;

submitter keyword: Weaning Piglet, Nutrition, Enterococcus lactis, LC-MS Proteomics, Probiotics,Microalgae, Chlorella vulgaris, Animal Science, Enterococcus faecium

André Almeida
contact affiliation	Departamento de Produção Animal Instituto Superior de Agronomia Tapada da Ajuda 1349-017 Lisboa Portugal
contact email	aalmeida@isa.ulisboa.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062608
     1. Label: PRIDE project
     2. Name: Proteome of liver, intestine and muscle of weaning piglets in response to dietary inclusion of Chlorella vulgaris or its combination with Enterococcus lactis probiotic