PXD062603 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MdpS and MdpS2 structurally and functionally diverge to regulate Streptococcus oralis mucin degradation |
| Description | The human oral cavity harbors a diverse microbial community, with oral streptococci, particularly the Streptococcus mitis group, playing a pivotal role in biofilm formation and oral health. Among these, Streptococcus oralis is a key early colonizer that stabilizes oral biofilms. Here, we identify two mucin-degrading proteases, MdpS and MdpS2, that enable S. oralis to degrade MUC5B, the sole gel-forming mucin in saliva. Despite low sequence similarity, these enzymes share a high degree of tertiary structural resemblance and exhibits complementary biological functions. Their activity leads to extensive MUC5B degradation influencing biofilm dynamics by promoting biofilm dispersal and altering MUC5B and/or MUC5AC BCi mucus gels properties, with MdpS2 displaying specificity for MUC5B gels. Our findings reveal a specialized role in biofilm structural remodeling, offering potential avenues for clinical applications in biofilm modulation and mucus degradation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-10 |
| AnnouncementXML | Submission_2025-11-09_16:30:46.262.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Liisa Arike |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-04 06:07:08 | ID requested | |
| ⏵ 1 | 2025-11-09 16:30:47 | announced | |
Publication List
| 10.1080/20002297.2025.2571186; |
| Leo F, Nilsson J, Arike L, Kumar S, Hilton E, Lood R, Thornton DJ, Duncan GA, Svens, ä, ter G, Wickstr, ö, m C, . J Oral Microbiol, 17(1):2571186(2025) [pubmed] |
Keyword List
| submitter keyword: MUC5B,Streptococcus oralis, biofilm dynamics, mucin degrading proteases, Oral microbiome, mucin degradation |
Contact List
| Fredrik Leo |
|---|
| contact affiliation | Department of Oral Biology and Pathology, Faculty of Odontology, Malmö University, Malmö, Sweden Genovis AB, Kävlinge, Sweden |
| contact email | fredrik.leo@mau.se |
| lab head | |
| Liisa Arike |
|---|
| contact affiliation | PhD |
| contact email | liisaarike@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062603
- Label: PRIDE project
- Name: MdpS and MdpS2 structurally and functionally diverge to regulate Streptococcus oralis mucin degradation
