PXD062592 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Thermal Proteome Analysis of nutrient stress in Cupriavidus necator expression dataset |
| Description | The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources or of autotrophic growth using CO2 fixation with H2 as an energy source. Under stress conditions, it is capable of producing biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total biomass. This metabolic versatility means that C. necator is under intense study for sustainable biotechnology processes; however a relative lack of understanding of the overall regulatory architecture has limited its application. The major mechanisms by which proteins can respond to shifting cellular demands are protein expression change and/or allosteric regulation. Here we use two powerful proteomics methods to investigate these responses in C. necator cells grown on balanced and low nitrogen (PHA-inducing) media. Using quantitative proteomics and protein stability analysis (which can report on conformation change), we find that proteins across different pathways respond through one or both of these regulatory modes, including coordinated adaption to nutrient stress by the PHA pathway, the Calvin cycle, and ribosomal proteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-10 |
| AnnouncementXML | Submission_2025-11-09_16:36:52.214.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD062592 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Eugene Dillon |
| SpeciesList | scientific name: Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha); NCBI TaxID: NEWT:381666; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-04 00:56:54 | ID requested | |
| ⏵ 1 | 2025-11-09 16:36:53 | announced | |
Publication List
| McKeever K, Tham JL, Bruch M, Narancic T, Connor KO, Upadhya SR, Ryan C, Dillon ET, Wynne K, Cagney G, Response to Nutrient Stress in the Industrial Model Bacterium Cupriavidus necator: A Thermal Proteome Profiling (TPP) Investigation. Proteomics, 25(20):25-38(2025) [pubmed] |
| 10.6019/PXD062592; |
| 10.1002/pmic.70045; |
Keyword List
| submitter keyword: Expression Proteomics,Thermal Proteomics |
Contact List
| Gerard Cagney |
|---|
| contact affiliation | University College Dublin, School of Biomolecular and Biomedical Science, Conway Institute Belfield Dublin 4 |
| contact email | gerard.cagney@ucd.ie |
| lab head | |
| Eugene Dillon |
|---|
| contact affiliation | UCD |
| contact email | eugene.dillon@ucd.ie |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD062592 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062592
- Label: PRIDE project
- Name: Thermal Proteome Analysis of nutrient stress in Cupriavidus necator expression dataset
