PXD062589-1

PXD062589 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Improving cellular fitness of human stem cell-derived islets under hypoxia
Description	Stem cell-derived islet cell therapy can effectively treat type 1 diabetes, but its efficacy is hindered by low oxygen supply post-transplantation, particularly in subcutaneous spaces and encapsulation devices, leading to cell dysfunction. The response to hypoxia and effective strategies to alleviate its detrimental effects remain poorly understood. Here, we show that beta cells within stem cell-derived islets gradually undergo a decline in cell identity and metabolic function in hypoxia. This is linked to reduced expression of immediate early genes (EGR1, FOS, and JUN), which downregulates key beta cell transcription factors. We further identified genes important for maintaining beta cell fitness in hypoxia, with EDN3 as a potent player. Elevated EDN3 expression preserves beta cell identity and function in hypoxia by modulating genes involved in beta cell maturation, glucose sensing and regulation. These insights improve the understanding of hypoxias impact on stem cell-derived islets, offering a potential intervention for clinical applications.
HostingRepository	MassIVE
AnnounceDate	2025-04-04
AnnouncementXML	Submission_2025-04-04_00:23:06.828.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shlomi Brielle
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	TMTpro
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 23:59:33	ID requested
⏵ 1	2025-04-04 00:23:07	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: hypoxia beta cells pancreas sc-islets, DatasetType:Proteomics

submitter keyword: hypoxia beta cells pancreas sc-islets, DatasetType:Proteomics

Contact List

Doug Melton
contact affiliation	Harvard University
contact email	dmelton@harvard.edu
lab head
Shlomi Brielle
contact affiliation	Harvard University
contact email	shlomi.brielle@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097523/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097523/