PXD062584-1

PXD062584 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicles of Emergomyces africanus modulate immune responses and reflect metabolic adaptations to nutrient availability
Description	Emergomyces africanus is a thermally dimorphic fungus and one of the leading causes of emergomycosis, a neglected infection primarily affecting immunocompromised individuals. Despite its clinical relevance, how E. africanus adapts to the host environment remains unknown. Recent studies suggest that extracellular vesicles (EVs) may play a key role during host adaptation by modulating immune responses and transporting virulence factors. It remains unknown whether E. africanus produces EVs. Here, we demonstrated EV production in E. africanus and investigated their size and composition after cultivation in nutrient-rich and nutrient-limited media, mimicking environmental and host-like conditions. In addition, we evaluated the effect of E. africanus EVs released in nutrient-limited media on bone marrow-derived dendritic cells (BMDCs) and bone marrow-derived macrophages (BMDMs). Under nutrient limitation, E. africanus released EVs enriched in proteins that, in other models, are virulence-associated, including catalase, HSP60, and chitinase, whereas EVs from rich media carried proteins linked to anabolic pathways. The presence of -1,3-glucans and a higher content of chitin-like structures were also detected in EVs released in nutrient-limited conditions. EVs from nutrient-limited conditions activated BMDCs, increasing MHC-II and CD40 expression and promoting a pro-inflammatory cytokine profile (IL-6 and TNF-α). In contrast, BMDMs exhibited elevated IL-10 levels, suggesting an anti-inflammatory shift. Remarkably, EV pre-treatment enhanced BMDM antifungal activity, significantly reducing E. africanus viability post-infection. These findings show that E. africanus dynamically adjust their EVs cargo in response to environmental cues, directly influencing immune modulation and fungal survival. Indeed, pre-treatment of the insect Galleria mellonella with these EVs induced a protective response against a lethal inoculum of Histoplasma capsulatum. This study reinforces the potential of EVs as therapeutic targets and offers new insights into how E. africanus adapts to the host environment.
HostingRepository	PRIDE
AnnounceDate	2026-02-05
AnnouncementXML	Submission_2026-02-05_10:11:07.841.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Sidoli
SpeciesList	scientific name: Emergomyces africanus; NCBI TaxID: NEWT:1955775;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 13:57:30	ID requested
⏵ 1	2026-02-05 10:11:08	announced

Dataset with its publication pending

submitter keyword: Emergomyces africanus, nutrient limitation, extracellular vesicles, immune modulation, macrophages, dendritic cells

Simone Sidoli
contact affiliation	Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, United States
contact email	simone.sidoli@einsteinmed.edu
lab head
Simone Sidoli
contact affiliation	Albert Einstein College of Medicine
contact email	simone.sidoli@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062584
     1. Label: PRIDE project
     2. Name: Extracellular vesicles of Emergomyces africanus modulate immune responses and reflect metabolic adaptations to nutrient availability