PXD062559-1

PXD062559 is an original dataset announced via ProteomeXchange.

Title	Site-specific profiling of structure and function of IgM B cell receptor glycans
Description	Although N-linked glycans play pivotal roles in the regulation of antibody effector functions, little is known about the composition and functional impact of glycans expressed by human B cell receptors (BCRs), likely due to technical challenges. Here, we describe the site-specific glycosylation profiles of all four N-linked glycosylation-sites of human IgM BCRs from primary naive and memory B cells. We show that the BCR glycans have not undergone structural changes during the transition from naive to memory cells. Moreover, using B cell lines expressing well-defined BCRs, we show that individual glycosylation sites are non-essential for cell-surface expression, and that the absence of the IgM BCR N209 glycan reduces the antigen-binding capacity of B cells. Collectively, these findings shows high conservation of IgM BCR glycosylation across IgM BCR B cell subsets and indicate a limited contribution to BCR expression and function, suggesting BCR glycans may have evolved to support IgM antibody functions.
HostingRepository	PRIDE
AnnounceDate	2025-12-09
AnnouncementXML	Submission_2025-12-09_05:27:10.454.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rayman Tjokrodirijo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 04:56:03	ID requested
⏵ 1	2025-12-09 05:27:10	announced

Dataset with its publication pending

submitter keyword: IgM, Primary naïve and memory B cells, N-linked glycosylation,Human B cell receptors

Peter A. van Veelen
contact affiliation	Head Proteomics Group, Leiden University Medical Center, Center for Proteomics and Metabolomics, PO Box 9600, Postal zone P1-Q, 2300 RC Leiden, The Netherlands
contact email	P.A.van_Veelen@lumc.nl
lab head
Rayman Tjokrodirijo
contact affiliation	Leiden University Medical Center, Proteomics group
contact email	r.t.n.tjokrodirijo@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD062559

PRIDE project URI

• PRIDE
  1. PXD062559
     1. Label: PRIDE project
     2. Name: Site-specific profiling of structure and function of IgM B cell receptor glycans