PXD062555-1

PXD062555 is an original dataset announced via ProteomeXchange.

Title	Identification and Characterisation of Taxonomically Informative Peptides
Description	Hair fibers serve as a prevalent and valuable form of biological trace evidence. Taxonomic classification of hair recovered during a criminal investigation provides crucial forensic intelligence and aids in evidence prioritisation. Traditional classification methods rely heavily on the analysis of hair shaft morphology, which requires individual analysis, can have limited taxonomic resolution, and is difficult to scale. Mass spectrometry-based proteomics can provide a biomolecular alternative by use of taxonomic amino acid polymorphisms to resolve the species of origin of trace evidence. In this study a proteomics workflow was developed for the discovery and characterization of taxonomically diagnostic hair peptides. Taxa were selected with a focus on relevance to crime scene investigation. A panel of 226 taxonomically informative peptides were identified and characterized, 209 of which are novel to this study. The biomarker panel includes 59 peptides with apparent single-species resolution, with remaining biomarkers being diagnostic in combination. The study also included 3 positive control keratin sequences where at least 2 are documented to occur in all mammals. The panel of taxa-discriminating markers was subsequently integrated into a forensic workflow, optimized for universal sample preparation and standardised LC/MS/MS data acquisition. The developed workflow was used to analyze single fur-hair shafts (20 mm), from four individuals from each species of interest. The data was further processed using a curated protein database, an efficient and validated bioinformatics workflow. The method was able to classify all hairs analysed with genus or species level resolution without prior source information.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_11:15:28.854.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062555
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romy Keane
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Capra hircus (Goat); NCBI TaxID: NEWT:9925; scientific name: Cavia porcellus (Guinea pig); NCBI TaxID: NEWT:10141; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825; scientific name: Vicugna pacos; NCBI TaxID: NEWT:30538; scientific name: Camelus dromedarius (Dromedary) (Arabian camel); NCBI TaxID: NEWT:9838; scientific name: Felis catus (Cat) (Felis silvestris catus); NCBI TaxID: NEWT:9685; scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Vulpes vulpes (Red fox); NCBI TaxID: NEWT:9627; scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: NEWT:9615; scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: NEWT:9986; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; scientific name: Ovis aries; NCBI TaxID: NEWT:9940;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-03 02:52:07	ID requested
⏵ 1	2026-05-13 11:15:30	announced

10.1021/acs.jproteome.5c00638;

10.6019/PXD062555;

Keane RE, Gummer JPA, Tidy RJ, Parker GJ, Taxonomic Source Identification of Hair. J Proteome Res, ():(2026) [pubmed]

submitter keyword: HRMS, Discovery proteomics,Taxonomic Classification

Joel Gummer
contact affiliation	Forensic Science Laboratory, Chemcentre, Western Australia
contact email	Jgummer@chemcentre.wa.gov.au
lab head
Romy Keane
contact affiliation	Chemcentre
contact email	r.keane@ecu.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062555
     1. Label: PRIDE project
     2. Name: Identification and Characterisation of Taxonomically Informative Peptides