PXD062536-1

PXD062536 is an original dataset announced via ProteomeXchange.

Title	High-performance proteomics using nano-, capillary- and micro-flow chromatographic separations
Description	In this study, we systematically evaluated the performance of μLC (50, 10 μL/min), capLC (5, and 1.5 μL/min), and nLC (0.3 μL/min ) flow-rates for proteome analysis using a Vanquish Neo HPLC coupled with a Q Exactive HF-X mass spectrometer. Serial dilutions of HeLa cell line digests were used for benchmarking the different setups and we intentionally fixed the total analysis time from injection-to-injection time of 60 minutes (24 samples per day). Showcase studies were also done on lable-free phospo-proteomics and kinobeads pull-down experiments.
HostingRepository	PRIDE
AnnounceDate	2025-09-04
AnnouncementXML	Submission_2025-09-04_03:15:39.046.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	giorgi tsiklauri
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-02 14:16:34	ID requested
⏵ 1	2025-09-04 03:15:39	announced

10.1021/ACS.JPROTEOME.5C00327;

submitter keyword: kinobeads, LC-MS, phospho-proteomics,capillary-flow chromatography

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Germany
contact email	kuster@tum.de
lab head
giorgi tsiklauri
contact affiliation	Technical University of Munich
contact email	gio.tsiklauri@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062536

PRIDE project URI

• PRIDE
  1. PXD062536
     1. Label: PRIDE project
     2. Name: High-performance proteomics using nano-, capillary- and micro-flow chromatographic separations