PXD062524 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | APEX2 proximity labelling of FRS2 interactors in UW228 cells |
| Description | The scaffold protein FRS2 is overexpressed in medulloblastoma (MB) and correlates with aggressive tumor phenotype and poor prognosis. Studies regarding FRS2's interactome have been limited to the assembly of the well described canonical FGFR signalosome. Here, we explored the proteomic proximity of FRS2 in MB cells exposed to serum-free medium conditions, stimulation with bFGF or treatment with the FRS2 small molecule ligand C7. Towards this aim, we expressed APEX2 fused to FRS2 in UW228 cells and stimulated labeling activity by co-incubation with biotinphenol and hydrogen peroxide. In the control setup, we expressed a FRS2-mNeonGreen fusion without a peroxidase enzymatic function. After labling and streptavidin purification of biotinylated proteins, we performed mass spectrometry and quantified relative abundances compared to control conditions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-06_16:13:42.132.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Martin Baumgartner |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | biotinylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-04-02 06:36:24 | ID requested | |
| ⏵ 1 | 2026-09-06 16:13:43 | announced | |
Publication List
| Kopp LL, Versamento D, Ciraulo B, Sch, ö, nholzer MT, Hochuli D, Lin MS, Baumgartner M, Fibroblast growth factor receptor substrate 2 interactome mapping reveals novel candidate interactors associated with migration and invasion. Cell Commun Signal, 24(1):(2026) [pubmed] |
| 10.1186/s12964-026-02943-8; |
Keyword List
| ProteomeXchange project tag: string |
| submitter keyword: Human, Medulloblastoma, Interactors, APEX2, FRS2 |
Contact List
| Martin Baumgartner |
|---|
| contact affiliation | University Children's Hospital Zurich |
| contact email | Martin.Baumgartner@kispi.uzh.ch |
| lab head | |
| Martin Baumgartner |
|---|
| contact affiliation | University Children's Hospital Zürich |
| contact email | martin.baumgartner@kispi.uzh.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062524
- Label: PRIDE project
- Name: APEX2 proximity labelling of FRS2 interactors in UW228 cells
