PXD062520-1

PXD062520 is an original dataset announced via ProteomeXchange.

Title	Genome-wide CRISPR screens and quantitative proteomics reveal remodelling of the aryl hydrocarbon receptor-controlled proteome through PARP7 activity
Description	PARP7 is an enzyme that uses donor substrate NAD+ to attach a single ADP-ribose moiety onto proteins related to immunity, transcription, and cell growth and motility. Despite the importance of PARP7 in these processes, PARP7 signalling networks remain under-researched. Here, we used genome-wide CRISPR screens and multiplex quantitative proteomics in distinct lung cancer cell lines treated with a PARP7 inhibitor to better understand PARP7 molecular functions. We discover that manipulating the aryl hydrocarbon receptor (AHR) transcriptional activity mediates PARP7 inhibitor sensitivity and triggers robust changes to the AHR-controlled proteome (AHR-ome). One of the striking features of such AHR-ome remodelling was the downregulation of Filamins A and B concurrent with the induction of the corresponding E3 ubiquitin ligase ASB2. We also show that suppressor of cytokine signalling 3 (SOCS3) reciprocally crosstalks to AHR. Inhibition of PARP7 in SOCS3 knockout cells leads to reduced viability compared to wild type cells treated with a PARP7 inhibitor. Our results reveal new signalling interplay among PARP7, AHR and SOCS3, and establish a useful resource to study the role of PARP7 in the regulation of AHR signalling and innate immunity through its ADP-ribosyl transferase activity.
HostingRepository	PRIDE
AnnounceDate	2025-05-11
AnnouncementXML	Submission_2025-05-11_04:19:56.147.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Eclipse; Orbitrap Astral; Orbitrap Exploris 480; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-02 05:25:47	ID requested
⏵ 1	2025-05-11 04:19:56	announced

Dataset with its publication pending

submitter keyword: screen, proteomics, AHR, mass spectrometry, PARP, CRISPR, ADP-ribosylation,aryl hydrocarbon receptor

Ivan Ahel
contact affiliation	University of Oxford Sir William Dunn School of Pathology Oxford OX1 3RE, UK
contact email	ivan.ahel@path.ox.ac.uk
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062520

PRIDE project URI

• PRIDE
  1. PXD062520
     1. Label: PRIDE project
     2. Name: Genome-wide CRISPR screens and quantitative proteomics reveal remodelling of the aryl hydrocarbon receptor-controlled proteome through PARP7 activity