PXD062518-1

PXD062518 is an original dataset announced via ProteomeXchange.

Title	STN1 facilitates metastasis by promoting transcription of EMT-activator ZEB1 in pancreatic cancer
Description	As pancreatic ductal adenocarcinoma (PDAC) poses a formidable clinical challenge, demanding further exploration of its pathogenesis and drugs targeted for metastasis-the main cause of mortality. Here, we identified STN1, a CST complex member crucial for maintaining telomere lengths and genome stability, as a key factor in promoting PDAC metastasis. Elevated STN1 levels correlate with poor patient survival, with oncogenic protein HOXB7 as an upstream transcription factor regulating STN1. Utilizing multiple PDAC experimental models, we discovered STN1’s role in promoting metastasis by acting as a novel upstream factor in epithelial-mesenchymal transition (EMT). Mechanistically, our proposed model suggests that during transcription, STN1 binds to displaced single-stranded DNA adjacent to the R-loop, recruiting STAT3 to activate ZEB1 transcription independent of its known telomere maintenance function. Notably, STAT3 inhibitors show enhanced efficiency in restraining metastatic potential in STN1-overexpressed PDAC cells, offering promise as a therapeutic avenue for targeting metastasis in STN1-overexpressed PDAC patients facing an unfavorable prognosis.
HostingRepository	PRIDE
AnnounceDate	2025-08-05
AnnouncementXML	Submission_2025-08-05_02:05:44.760.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-02 05:14:23	ID requested
⏵ 1	2025-08-05 02:05:45	announced

Dataset with its publication pending

submitter keyword: pancreatic cancer

STN1

STAT3

Yuxin Yin
contact affiliation	Department of Pathology, Institute of Systems Biomedicine, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking University Health Science Center, Beijing, China
contact email	yinyuxin@hsc.pku.edu.cn
lab head
Zhe Zhou
contact affiliation	Peking University
contact email	zzhou24@pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD062518

PRIDE project URI

• PRIDE
  1. PXD062518
     1. Label: PRIDE project
     2. Name: STN1 facilitates metastasis by promoting transcription of EMT-activator ZEB1 in pancreatic cancer