PXD062500-1

PXD062500 is an original dataset announced via ProteomeXchange.

Title	Differential Proteomics of pprI-Knockout Deinococcus radiodurans: A Label-Free Quantitative Analysis
Description	Deinococcus radiodurans, renowned for its exceptional resistance to radiation, provides a robust model for elucidating cellular stress responses and DNA repair mechanisms. The global regulator PprI plays a pivotal role in maintaining this resilience by coordinating DNA damage repair, antioxidant defense, and metabolic reprogramming. This study employs label-free quantitative proteomics coupled with high-resolution mass spectrometry to systematically compare the proteomic profiles of pprI-knockout and wild-type D. radiodurans strains. Under stringent screening criteria, we identified 719 significantly upregulated and 281 significantly downregulated proteins in the knockout strain. Functional analysis revealed that PprI deletion disrupts homologous recombination (HR) repair, activates nucleotide excision repair (NER) and base excision repair (BER) as a compensatory mechanism, and impairs Mn/Fe homeostasis and carotenoid biosynthesis, leading to increased oxidative stress. Furthermore, PprI deficiency induces significant metabolic reprogramming, including impaired purine synthesis, compromised cell wall integrity,etc. These findings highlight PprI as a central orchestrator of multi-pathway synergies, providing critical insights into the molecular mechanisms underlying extreme radiation resistance.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_16:08:10.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	siyu Zhu
SpeciesList	scientific name: Deinococcus radiodurans; NCBI TaxID: 1299;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-01 22:44:45	ID requested
⏵ 1	2025-06-29 16:08:11	announced

10.3390/proteomes13020019;

Zhu S, Liu F, Wang H, Zhang Y, Through Label-Free Quantitative Proteomics. Proteomes, 13(2):(2025) [pubmed]

submitter keyword: Radiation response metalloprotease

Label-free quantitative proteomics

DNA damage repair

Antioxidant defense

Metabolic regulation

Yongqian Zhang
contact affiliation	Beijing Institute of Technology
contact email	zyq@bit.edu.cn
lab head
siyu Zhu
contact affiliation	Beijing Institute of Technology
contact email	1120222466@bit.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062500
     1. Label: PRIDE project
     2. Name: Differential Proteomics of pprI-Knockout Deinococcus radiodurans: A Label-Free Quantitative Analysis