PXD062427-1

PXD062427 is an original dataset announced via ProteomeXchange.

Title	Phosphate refeeding in Arabidopsis thaliana seedlings exerts differential impacts on the shoot and root proteome.
Description	Phosphorus (P) is an essential plant macronutrient required for fundamental biochemical, metabolic, and physiological processes. In its inorganic and chemically available form of orthophosphate (H2PO4-; Pi), plant root epidermal cells are able to directly assimilate this mineral from the soil via plasmalemma Pi transporters (Dissanayaka et al., 2021). The role of Pi in plants is multifaceted; it serves as a central structural component of important biomolecules, including nucleic acids, sugar phosphates, and phospholipids, and is also required for important regulatory processes such as photosynthesis (i.e. triose-P exchange) and respiration. Additionally, Pi allosterically regulates several enzymes of central plant metabolism via covalent attachment to a specific amino acid residue (i.e., phosphorylation). Despite its significance, most soil-Pi levels are extremely suboptimal for sustaining crop growth due its precipitation and subsequent formation of metal cation-Pi complexes (Chen & Liao, 2016). Furthermore, a significant amount of soil-Pi is trapped in organic substrates (e.g. decaying biomatter) which must be mineralized prior to plant uptake (Raghothama, 1999). Typical concentrations of readily available soil-Pi (0.1-10 μM) are thousands of orders of magnitude lower than those found in nutrient-sufficient plant tissue, and as a result, excessive quantities of unsustainable Pi fertilizers sourced from finite ‘rock-Pi’ reserves are applied to crops (Hinsinger, 2001; Raghothama, 1999; Blackwell et al., 2019). In addition to the rapidly depleting levels of global rock-Pi reserves, excess Pi runoff from these fertilizers can leach into aquatic ecosystems causing eutrophication and harmful algal blooms (Lambers & Plaxton, 2015). Given the inefficiency of Pi fertilizers and scarcity of Pi reserves, agricultural solutions are urgently needed to reduce our over-reliance on exogenous Pi application. By studying the adaptations of -Pi plants, we may uncover potential biological targets for engineering Pi-efficient crop varieties.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:35:50.030.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Uhrig
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-31 15:29:11	ID requested
⏵ 1	2026-02-08 16:35:51	announced

10.1111/pce.70311;

Smith MA, Grubb LE, Benidickson KH, Mehta D, Plaxton WC, Uhrig RG, Phosphate Resupply Differentially Impacts the Shoot and Root Proteomes of Arabidopsis thaliana Seedlings. Plant Cell Environ, 49(3):1598-1616(2026) [pubmed]

submitter keyword: Arabidopsis thaliana, Shoots & Roots, Phosphate Starvation, Proteomics

R. Glen Uhrig
contact affiliation	Department of Biological Sciences, University of Alberta, Canada
contact email	ruhrig@ualberta.ca
lab head
Richard Uhrig
contact affiliation	University of Alberta
contact email	ruhrig@ualberta.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD062427

PRIDE project URI

• PRIDE
  1. PXD062427
     1. Label: PRIDE project
     2. Name: Phosphate refeeding in Arabidopsis thaliana seedlings exerts differential impacts on the shoot and root proteome.