PXD062419-1

PXD062419 is an original dataset announced via ProteomeXchange.

Title	Peptidomic analysis of CSF reveals new biomarker candidates for amyotrophic lateral sclerosis
Description	Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease and novel biomarkers are needed. We applied mass-spectrometry-based peptidomic analysis in cerebrospinal fluid (CSF) samples of ALS and non-neurodegenerative control patients (Con) from a discovery (n=48) and validation (n=109) cohort for biomarker discovery. We identified 33605 peptides in CSF samples from the discovery cohort. Systematic selection for the best candidates revealed a targeted method with eight peptides derived from seven proteins. In the validation cohort, NFL, MAP1B, MYL1, APOC1 peptides were up-regulated and peptides from CADM3, SCG1 and PENK down-regulated in ALS compared to Con. Combination of all peptides in a logistic regression model led to an area under the curve value of 98% for the discrimination of ALS from controls. Data of the NFL peptide strongly correlated with an established NFL immunoassay (Ella, r=0.97). The peptide biomarker candidates are derived from proteins with different function and their determination with our method provides the opportunity for simultaneous investigation of key processes in ALS and other neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2025-06-28
AnnouncementXML	Submission_2025-06-28_06:10:02.420.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062419
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Besnik Muqaku
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-31 09:51:35	ID requested
⏵ 1	2025-06-28 06:10:02	announced

10.6019/PXD062419;

submitter keyword: Human, LC-MSMS, Peptidomics, ALS, CSF, Neurodegeneration, Biomarker

Patrick Oeckl
contact affiliation	German Center for Neurodegenerative Diseases (DZNE e.V.), Ulm, Germany Department of Neurology, Ulm University Hospital, Ulm, Germany
contact email	patrick.oeckl@uni-ulm.de
lab head
Besnik Muqaku
contact affiliation	German Center for Neurodegenerative Diseases (DZNE)
contact email	besnik.muqaku@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD062419

PRIDE project URI

• PRIDE
  1. PXD062419
     1. Label: PRIDE project
     2. Name: Peptidomic analysis of CSF reveals new biomarker candidates for amyotrophic lateral sclerosis