PXD062405-1

PXD062405 is an original dataset announced via ProteomeXchange.

Title	RHBDL2 Drives Lipid Metabolic Reprogramming in Osteosarcoma via USP3-Mediated Deubiquitination of PPT1
Description	Osteosarcoma (OS), a highly malignant bone tumor, is characterized by rapid progression and poor prognosis, with lipid metabolic reprogramming playing a crucial role in its pathogenesis. This study investigates the role of RHBDL2 in OS progression and its therapeutic potential. We demonstrate that RHBDL2 is overexpressed in OS tumor tissues, correlating with poor prognosis. RHBDL2 suppression significantly inhibits OS cell proliferation, migration, and lipid metabolism while enhancing apoptosis. Mechanistically, RHBDL2 interacts with USP3, enhancing its expression to stabilize PPT1 by removing ubiquitin chains, thereby promoting lipid synthesis and storage. Importantly, we identified Epigallocatechin gallate (EGCG) as a small-molecule inhibitor that disrupts the RHBDL2/USP3 interaction, potentially offering a therapeutic strategy to inhibit osteosarcoma progression without affecting physiological bone remodeling.
HostingRepository	iProX
AnnounceDate	2025-03-31
AnnouncementXML	Submission_2026-03-31_18:01:37.587.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kewei Zhu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-31 05:01:00	ID requested
⏵ 1	2026-03-31 18:01:38	announced

Dataset with its publication pending

submitter keyword: osteosarcoma, Lipid metabolic reprogramming, RHBDL2

Kewei Zhu
contact affiliation	The Second Xiangya Hospital of Central South University
contact email	25325188@qq.com
lab head
Kewei Zhu
contact affiliation	The Second Xiangya Hospital of Central South University
contact email	hyan44@outlook.com
dataset submitter

iProX dataset URI