PXD062375 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | KRAS-Dependent Glycolytic Reprogramming of Endothelial Cells in Sporadic Arteriovenous Malformations |
| Description | Brain arteriovenous malformation (bAVM) is a disease characterized by the formation of tangled blood vessels with direct connections between arteries and veins. There is currently limited understanding of the underlying molecular mechanisms that are responsible for the development of bAVM. Our previous work identified the presence of somatic activating KRAS mutations in endothelial cells (ECs) isolated from clinical bAVM tissue. As such, we performed this proteomics study to profile the molecular impact of mutant KRAS expression on endothelial biology. We engineered a human EC model with doxycycline-inducible expression of mutant KRAS G12V. As mutant KRAS ECs preferentially activated the MAPK/ERK signaling cascade with no change to the activities of PI3K/AKT or MAPK/p38 pathways, we also included a MEK inhibitor (SL327) in this study to delineate MEK-dependent versus MEK-independent proteome changes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-14 |
| AnnouncementXML | Submission_2025-12-14_09:53:21.677.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD062375 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ruilin Wu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-29 11:46:29 | ID requested | |
| ⏵ 1 | 2025-12-14 09:53:22 | announced | |
Publication List
Keyword List
Contact List
| Jason E. |
|---|
| contact affiliation | Toronto General Hospital Research Institute, University Health Network; Toronto, Ontario, Canada; Temerty Faculty of Medicine, University of Toronto; Toronto, Ontario, Canada; Peter Munk Cardiac Centre, University Health Network; Toronto, Ontario, Canada. |
| contact email | jason.fish@utoronto.ca |
| lab head | |
| Ruilin Wu |
|---|
| contact affiliation | University of Toronto |
| contact email | ruiw251@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD062375 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062375
- Label: PRIDE project
- Name: KRAS-Dependent Glycolytic Reprogramming of Endothelial Cells in Sporadic Arteriovenous Malformations
