PXD062366-1

PXD062366 is an original dataset announced via ProteomeXchange.

Title	Alterations in tear proteomes of adults with pre-diabetes and type 2 diabetes mellitus but without diabetic retinopathy
Description	This study aims to explore the impact of type 2 diabetes mellitus (T2DM) on tear proteome, specifically investigating whether alterations occur before the development of diabetic retinopathy. Flush tear samples were collected from healthy subjects (n=13), subjects with preDM (n=16) and T2DM (n=18). Tear proteins were processed and analyzed by mass spectrometry-based shotgun proteomics using data independent acquisition parallel acquisition serial fragmentation (diaPASEF) approach. Machine learning algorithms, including random forest, lasso regression, and support vector machine, and statistical tools were used to identify biomarkers. Machine learning models identified 17 proteins with high importance in classification. Among these, five proteins (cystatin-S, S100-A11, submaxillary gland androgen-regulated protein 3B, immunoglobulin lambda variable 3-25 and lambda constant 3) exhibited differential expression across these three groups. No correlations were identified between proteins and clinical assessments of the ocular surface. Notably, the 17 important proteins identified by machine learning showed su-perior prediction accuracy in distinguishing all three groups (healthy, preDM, and T2DM) compared to the five proteins that were statistically differentially expressed. Alterations in tear proteome profile were observed in adults with preDM and T2DM before the clinical diagnosis of ocular abnormality including retinopathy.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:21:17.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoting Qin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-28 18:12:15	ID requested
⏵ 1	2025-08-03 16:21:18	announced

10.3390/proteomes13030029;

Qin G, Chao C, Duong S, Smith J, Lin H, Harrison WW, Cai C, Alterations in Tear Proteomes of Adults with Pre-Diabetes and Type 2 Diabetes Mellitus but Without Diabetic Retinopathy. Proteomes, 13(3):(2025) [pubmed]

submitter keyword: Tear film

type 2 diabetes mellitus

mass spectrometry

proteomics, machine learning.

Guoting Qin
contact affiliation	University of Houston
contact email	gqin@central.uh.edu
lab head
Guoting Qin
contact affiliation	University of Houston
contact email	gqin@central.uh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062366
     1. Label: PRIDE project
     2. Name: Alterations in tear proteomes of adults with pre-diabetes and type 2 diabetes mellitus but without diabetic retinopathy