PXD062353-1

PXD062353 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of expi293 conditioned media purified with immobilized metal affinity chromatography
Description	Isthmin-1 (ISM1) is a recently described adipokine with insulin-like properties, reported to regulate hyperglycemia and liver steatosis. However, contrary to earlier studies, we demonstrate that highly purified ISM1 does not stimulate AKT phosphorylation in 3T3-F442A pre-adipocytes. Instead, this activity is attributed to co-purifying factors. Using proteomic mass spectrometry, we analyzed the ISM1-free sample that exhibits activity on AKT phosphorylation in 3T3-F442A cells and identified the growth factors PDGF-AA and/or GDF15 as the likely contributors to this effect.
HostingRepository	PRIDE
AnnounceDate	2025-04-02
AnnouncementXML	Submission_2025-04-02_06:30:17.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenxue Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-28 09:42:21	ID requested
⏵ 1	2025-04-02 06:30:17	announced

Dataset with its publication pending

submitter keyword: DIA,Proteomics,AKT

Yansheng Liu
contact affiliation	Yale School of Medicine
contact email	yansheng.liu@yale.edu
lab head
Wenxue Li
contact affiliation	Yale University
contact email	wenxue.li@yale.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062353
     1. Label: PRIDE project
     2. Name: Proteomics analysis of expi293 conditioned media purified with immobilized metal affinity chromatography