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PXD062353-1

PXD062353 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis of expi293 conditioned media purified with immobilized metal affinity chromatography
DescriptionIsthmin-1 (ISM1) is a recently described adipokine with insulin-like properties, reported to regulate hyperglycemia and liver steatosis. However, contrary to earlier studies, we demonstrate that highly purified ISM1 does not stimulate AKT phosphorylation in 3T3-F442A pre-adipocytes. Instead, this activity is attributed to co-purifying factors. Using proteomic mass spectrometry, we analyzed the ISM1-free sample that exhibits activity on AKT phosphorylation in 3T3-F442A cells and identified the growth factors PDGF-AA and/or GDF15 as the likely contributors to this effect.
HostingRepositoryPRIDE
AnnounceDate2025-04-02
AnnouncementXMLSubmission_2025-04-02_06:30:17.601.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWenxue Li
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-28 09:42:21ID requested
12025-04-02 06:30:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DIA,Proteomics,AKT
Contact List
Yansheng Liu
contact affiliationYale School of Medicine
contact emailyansheng.liu@yale.edu
lab head
Wenxue Li
contact affiliationYale University
contact emailwenxue.li@yale.edu
dataset submitter
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Dataset FTP location
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