PXD062353 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics analysis of expi293 conditioned media purified with immobilized metal affinity chromatography |
Description | Isthmin-1 (ISM1) is a recently described adipokine with insulin-like properties, reported to regulate hyperglycemia and liver steatosis. However, contrary to earlier studies, we demonstrate that highly purified ISM1 does not stimulate AKT phosphorylation in 3T3-F442A pre-adipocytes. Instead, this activity is attributed to co-purifying factors. Using proteomic mass spectrometry, we analyzed the ISM1-free sample that exhibits activity on AKT phosphorylation in 3T3-F442A cells and identified the growth factors PDGF-AA and/or GDF15 as the likely contributors to this effect. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-02 |
AnnouncementXML | Submission_2025-04-02_06:30:17.601.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wenxue Li |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-03-28 09:42:21 | ID requested | |
⏵ 1 | 2025-04-02 06:30:17 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: DIA,Proteomics,AKT |
Contact List
Yansheng Liu |
contact affiliation | Yale School of Medicine |
contact email | yansheng.liu@yale.edu |
lab head | |
Wenxue Li |
contact affiliation | Yale University |
contact email | wenxue.li@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062353
- Label: PRIDE project
- Name: Proteomics analysis of expi293 conditioned media purified with immobilized metal affinity chromatography