PXD062347-1

PXD062347 is an original dataset announced via ProteomeXchange.

Title	Deceptive Ceropegia sandersonii uses an arabinogalactan for trapping its fly pollinators
Description	Many plant species have evolved surfaces designed to reduce insect attachment. Among such plants are deceptive trap flowers of Ceropegia. Their gliding zones consist of epidermal cells each topped by a papilla secreting a single small liquid droplet on its tip. So far, the molecular and physical mechanisms controlling the function of these droplets are unknown. We analyzed the droplets of C. sandersonii flowers by microscopic approaches, studied how they behave when getting in contact with the feet of fly pollinators, and analyzed their chemical composition. The droplets contaminate the insect feet, on which they solidify. As its main component a negatively charged polysaccharide containing a β1,3-galactan backbone and Rhaa1,4GlcAβ1,6[Arafa1,3]Galβ1,6 side chains or truncated versions of it was identified by NMR spectroscopy. The chemical structure represents a rudiment version of an arabinogalactan, which is supported by its binding to β-D-glucosyl Yariv reagent. A proteomics approach revealed candidates of arabinogalactan proteins to which the polysaccharide is connected. The high amount of GlcA in the polysaccharide explains the unusual physical characteristics of the droplets like viscoelasticity and hygroscopy. We add a new function to arabinogalactan proteins, and discuss, why the identified polymer is well suited for catching and temporarily trapping of pollinators.
HostingRepository	PRIDE
AnnounceDate	2025-04-11
AnnouncementXML	Submission_2025-04-11_05:30:21.997.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Regl
SpeciesList	scientific name: Ceropegia sandersonii; NCBI TaxID: 141469;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-28 07:46:34	ID requested
⏵ 1	2025-04-11 05:30:22	announced

Dataset with its publication pending

submitter keyword: NMR spectroscopy,anti-adhesive surface, peptide mapping, mass spectrometry, AGP, polysaccharide, Ceropegia sandersonii, monosaccharide composition, fly-trapping flower

Raimund Tenhaken
contact affiliation	Department of Environment and Biodiversity, University of Salzburg, Hellbrunnerstraße 34, 5020 Salzburg, Austria
contact email	raimund.tenhaken@plus.ac.at
lab head
Christof Regl
contact affiliation	University of Salzburg
contact email	christof.regl@sbg.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD062347

PRIDE project URI

• PRIDE
  1. PXD062347
     1. Label: PRIDE project
     2. Name: Deceptive Ceropegia sandersonii uses an arabinogalactan for trapping its fly pollinators