PXD062299-1

PXD062299 is an original dataset announced via ProteomeXchange.

Title	Boron Activated Covalent Modification and Micro-Dissection of Amyloid Deposits in Alzheimer’s Disease Brain Tissue
Description	Congo Red (CR) dye is historically used for histochemical staining of amyloid deposition in tissue. Its characteristic aryl azo moiety renders it chemically stable as a non-covalent pan-amyloid binder. Here, we discover in serendipity that the CR dye can covalently modify amyloid proteins by activation of the inert azo bond using neutral borate buffer at ambient temperature. We first show that boronic acid is responsible for azo bond-mediated protein bioconjugation. Furthermore, we also observe that ultraviolet light-induced azo trans-to-cis isomerization significantly enhances the labeling efficiency. Leveraging the discovered covalent chemistry, we apply CR-alkyne derivative with a click enrichment handle to label, capture and enrich amyloid deposits in Alzheimer’s disease (AD) mouse brain tissue. Finally, we identify the composition of amyloid deposits by proteomics profiling, revealing typical neurodegenerative diseases biomarkers, including MAPT and 14-3-3 family proteins. Overall, this work presents a new type of azo based bioconjugation chemistry previously known to be inert but herein activated by neutral borate buffer.
HostingRepository	PRIDE
AnnounceDate	2025-03-27
AnnouncementXML	Submission_2025-03-27_07:09:57.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huan Feng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-27 02:18:19	ID requested
⏵ 1	2025-03-27 07:09:58	announced

Dataset with its publication pending

submitter keyword: bioconjugation, azo compounds · boron chemistry· amyloid · Alzheimer’s disease

Huan Feng
contact affiliation	Dalian Institute of Chemical Physics
contact email	fenghuan@dicp.ac.cn
lab head
Huan Feng
contact affiliation	Dalian Institue of Chemical Physics
contact email	fenghuan@dicp.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062299
     1. Label: PRIDE project
     2. Name: Boron Activated Covalent Modification and Micro-Dissection of Amyloid Deposits in Alzheimer’s Disease Brain Tissue