PXD062288-2

PXD062288 is an original dataset announced via ProteomeXchange.

Title	Candidate Arabidopsis MYB5-interacting proteins in developing siliques identified by IP-MS
Description	2 g developing siliques were harvested from the pMYB5:MYB5-GFP/myb5-2 complementary plants treated with 100 μM GA3 or 50 μM PAC. The 35S:GFP transgenic plants served as a negative control. Siliques were immediately crosslinked with 1% paraformaldehyde for 30 min on ice in a vacuum chamber. Total proteins were extracted using the Plant Cell lysis buffer for Western and IP (Beyotime, P0043) supplemented with phenylmethylsulfonyl fluoride (Solabio) and a proteinase inhibitor cocktail (Calbiochem). To reduce non-specific binding, protein extracts were pre-cleared with 50 μL of Binding control Agarose beads (ChromTek) for 1 h at 4 °C with gentle rotation. After brief centrifugation, the supernatant was incubated with 50 μL of GFP-Trap Agarose beads (ChromTek) for 2 h at 4 °C with gentle rotation. After 10 washes of the beads with phosphate-buffered saline, proteins on the beads were eluted through boiling in SDS-PAGE sample buffer for 10 min and then briefly separated in 10% SDS-PAGE gel. The gel regions containing proteins were cut and then subjected to LC-MS/MS analysis at Applied Protein Technology (Zhejiang). Three biological replicates were performed. The MS/MS database search was performed using the Maxquant software against the NCBI database with the default parameters (FDR < 0.01).
HostingRepository	iProX
AnnounceDate	2025-03-27
AnnouncementXML	Submission_2025-05-19_00:19:21.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Xu
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-26 21:07:35	ID requested
1	2025-03-26 21:07:49	announced
⏵ 2	2025-05-19 00:19:21	announced	2025-05-19: Add Publication.

Xu Y, Du J, Hao R, Ma S, Ma Y, Zhou G, Hu R, Li S, Gibberellin signaling regulates pectin biosynthesis in Arabidopsis. Nat Commun, 16(1):4065(2025) [pubmed]

submitter keyword: Arabidopsis, MYB5, IP-MS

Yan Xu
contact affiliation	Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
contact email	xuyan@qibebt.ac.cn
lab head
Yan Xu
contact affiliation	Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
contact email	xuyan@qibebt.ac.cn
dataset submitter

iProX dataset URI