PXD062280-1

PXD062280 is an original dataset announced via ProteomeXchange.

Title	Single-vesicle heterogeneity and uptake of extracellular vesicles affected by oncogenic KRAS and BRAF revealed by proteomics and nano-flow cytometry
Description	This study explores the effects of KRAS and BRAF mutations on the heterogeneity, distribution, and uptake of extracellular vesicles (EVs), with a focus on their role in cancer biology and therapeutic potential. Nine cancer cell lines, including colorectal (Caco-2, HT29, WiDr, HCT116, LoVo) and lung cancer (H292, H1703, A549, H358), were selected based on KRAS and BRAF mutation status. EVs were isolated and characterized by TEM, Western blotting, and NTA. Proteomic analysis revealed that KRAS and BRAF mutant EVs were enriched in specific proteins, such as CD44 and LSR in KRAS mutations, and ITGB4 and CSPG4 in BRAF mutations. EV internalization was influenced by endocytosis and macropinocytosis, with competitive inhibition experiments showing the involvement of hyaluronic acid for CD44-mediated uptake and chondroitin sulfate for CSPG4. Finally, Doxorubicin-loaded KRAS-mutant EVs effectively delivered the drug to the nucleus, escaping lysosomal degradation. These findings suggest mutation-specific differences in EV biology and highlight their potential as targeted drug delivery vehicles in cancer therapy.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-30_23:03:40.174.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongsic Choi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-26 19:55:48	ID requested
⏵ 1	2026-08-30 23:03:40	announced
2	2026-08-31 17:10:23	announced	2026-09-01: Updated project metadata.

10.1038/S41598-026-65540-2;

10.1038/s41598-026-65540-2;

submitter keyword: BRAF mutation,Extracellular vesicles, KRAS mutation, lung cancer, colorectal cancer

Dongsic Choi
contact affiliation	Department of Biochemistry, Soonchunhyang University, College of Medicine, 31 Suncheonhyang 6-gil, Bongmyeong-dong, office# 310, Cheonan-si, Chungcheongnam-do, 31151, Republic of Korea
contact email	dongsic@sch.ac.kr
lab head
Dongsic Choi
contact affiliation	Department of Biochemistry, Soonchunhyang University, College of Medicine 31 Suncheonhyang 6-gil, Bongmyeong-dong, office# 310 Cheonan-si, Chungcheongnam-do, 31151
contact email	dongsic@sch.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD062280

PRIDE project URI

• PRIDE
  1. PXD062280
     1. Label: PRIDE project
     2. Name: Single-vesicle heterogeneity and uptake of extracellular vesicles affected by oncogenic KRAS and BRAF revealed by proteomics and nano-flow cytometry