PXD062276 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comprehensive Proteomic Profiling of Breast Cancer-Derived Small Extracellular Vesicles Unveiled PXDN and GGT5 as Novel Protein Markers Implicated in Oncogenic Signaling Networks |
| Description | Breast cancer (BC) is the most prevalent cancer and the second leading cause of cancer-related mortality among Canadian women. Early detection and treatment can significantly improve survival rates. The potential use of small extracellular vesicles (sEVs) as biomarkers for early BC diagnosis has gained increasing interest, mainly due to their promise as a non-invasive detection method. This study compared the sEVs proteome derived from a highly metastatic BC cell line, MDA-MB-231, with its parental cell line and exosomes derived from a non-cancerous breast epithelial MCF-10A cell line. Bioinformatic analyses revealed thatMDA-MB-231-derived sEVs showed enrichment pathway for cancer growth and proliferation. The proteins of these pathways can be a good source for BC biomarkers. In this study, three proteins were selected based on their unique presence in MDA-MB-231-derived sEVs and their contribution to the detected pathways related to BC: peroxidasin homolog (PXDN), glutathione hydrolase 5 proenzyme (GGT5), and plasminogen activator inhibitor 1 (SERPINE1). These proteins were validated using mass spectrometry-based parallel reaction monitoring. This study highlights the promise of sEV-based proteins as non-invasive biomarkers for early BC diagnosis, potentially improving detection methods and patient outcomes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-17 |
| AnnouncementXML | Submission_2025-11-17_14:08:39.173.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD062276 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Abdullah Khraibah |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | alkylated residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-26 18:55:20 | ID requested | |
| ⏵ 1 | 2025-11-17 14:08:39 | announced | |
Publication List
Keyword List
| submitter keyword: Human,Small extracellualr vesicles, Breast Cancer, MCF-10A , GGT5, MDA-MB-231, PXDN, LC-MS |
Contact List
| Maxim, Berezovski |
|---|
| contact affiliation | Department of Chemistry and Biomolecular Sciences, University of Ottawa, Canada (lab head) |
| contact email | Maxim.berezovski@uottawa.ca |
| lab head | |
| Abdullah Khraibah |
|---|
| contact affiliation | University of Ottawa |
| contact email | akhra046@uottawa.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD062276 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062276
- Label: PRIDE project
- Name: Comprehensive Proteomic Profiling of Breast Cancer-Derived Small Extracellular Vesicles Unveiled PXDN and GGT5 as Novel Protein Markers Implicated in Oncogenic Signaling Networks
