PXD062268-1

PXD062268 is an original dataset announced via ProteomeXchange.

Title	ULTRAPETALA1 modulates H3K27me3 and directly enhances POLYCOMB REPRESSIVE COMPLEX 2 activity for fine-tuned reproductive transitions
Description	The antagonistic POLYCOMB (PcG) REPRESSIVE COMPLEX 2 (PRC2) and trithorax (trxG) chromatin machineries play a major role in orchestrating gene expression during the development of multicellular eukaryotes. These complexes are well known for depositing and maintaining the repressive H3K27me3 and activating H3K4me3 marks, respectively. However, the mechanisms that govern the switch between these functions remains elusive, especially in plants, whose lifelong, flexible development relies heavily on this process. Here we demonstrate that the plant specific ULTRAPETALA1 (ULT1) protein, previously reported as a trxG factor that antagonizes the PRC2 enzymatic subunit CURLY LEAF (CLF), also exhibits a repressive function, increasing H3K27me3 levels at over a thousand genes. We discovered a physical interaction between ULT1 and PRC2 components, particularly the SWINGER (SWN) enzymatic subunit. We further show that ULT1 significantly enhances PRC2SWN enzymatic activity in vitro, corroborating our epigenomic and developmental genetic data that reveal different ULT1 activity depending on the catalytic subunit of the PRC2 complex. This study provides new insights into the relative activities of CLF and SWN and introduces a novel mechanistic framework for a chromatin switch mediated by a bivalent trxG/PcG factor.
HostingRepository	PRIDE
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-21_09:02:28.098.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD062268
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Astrid Bruckmann
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-26 12:34:40	ID requested
⏵ 1	2026-07-21 09:02:28	announced

10.1038/S41477-026-02363-Z;

10.6019/PXD062268;

submitter keyword: Arabidopsis, Q-TOF

Astrid Bruckmann
contact affiliation	University of Regensburg Biochemistry I Mass Spectrometry group
contact email	astrid.bruckmann@ur.de
lab head
Astrid Bruckmann
contact affiliation	University Regensburg
contact email	astrid.bruckmann@ur.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062268
     1. Label: PRIDE project
     2. Name: ULTRAPETALA1 modulates H3K27me3 and directly enhances POLYCOMB REPRESSIVE COMPLEX 2 activity for fine-tuned reproductive transitions