PXD062258-3

PXD062258 is an original dataset announced via ProteomeXchange.

Title	Unveiling previously undescribed circadian clock regulators and action mechanisms via TurboID-based profiling of the LWD1 interactome in Arabidopsis thaliana
Description	The synchronization of the internal circadian clock with the external 24-h diurnal cycle is critical for ensuring optimal plant growth fitness. Despite lacking a DNA binding domain, LIGHT-REGULATED WD proteins (LWDs) are essential for sustaining and fine-tuning circadian rhythm1,2. However, the action mechanisms of how LWDs orchestrate the clock gene expressions remain largely elusive. We systematically identified 219 potential LWD1 protein partners at three Zeitgeber times (ZTs) using TurboID-mediated proximity labeling approach. A few LWD1-interacting epigenetic regulators are verified as new regulators of the Arabidopsis circadian clock, flowering time, and light-inhibited hypocotyl elongation. Our data also supports a new working model for LWD1-PSEUDO RESPONSE REGULATORS (PRRs)-TOPLESS (TPL) complex to repress the morning gene CIRCADIAN CLOCK ASSOCIATED (CCA1) in the late afternoon under diel cycles, shaping the expression trough of CCA1 in the late afternoon. Our results revealed a new co-repressor role of LWD1 by working with epigenetic regulators and transcription factors to underpin the precise operation of the circadian clock.
HostingRepository	PRIDE
AnnounceDate	2026-07-22
AnnouncementXML	Submission_2026-07-21_23:20:41.266.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-Chih Hsu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	biotinylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-26 04:54:11	ID requested
1	2026-07-21 03:30:41	announced
2	2026-07-21 23:07:47	announced	2026-07-22: Updated project metadata.
⏵ 3	2026-07-21 23:20:41	announced	2026-07-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Circadian clock, Photomorphogenesis, TurboID, Proximality labeling, Photoperiodic flowering, LWD1

Shu-Hsing Wu
contact affiliation	Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
contact email	shuwu@as.edu.tw
lab head
Chuan-Chih Hsu
contact affiliation	Institute of Plant and Microbial Biology, Academia Sinica
contact email	pclipmb@gate.sinica.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD062258

PRIDE project URI

• PRIDE
  1. PXD062258
     1. Label: PRIDE project
     2. Name: Unveiling previously undescribed circadian clock regulators and action mechanisms via TurboID-based profiling of the LWD1 interactome in Arabidopsis thaliana