PXD062251-1

PXD062251 is an original dataset announced via ProteomeXchange.

Title	Tumor Microenvironment-Associated Oxidative Stress Impairs SIRT1 Secretion to Suppress Anti-Tumor Immune Response
Description	To further investigate the secretion process of SIRT1, we overexpressed FLAG-tagged WT and DEL forms of SIRT1 in HEK293 cells. SIRT1-interacting proteins were isolated from HEK293 cell extracts through co-immunoprecipitation (co-IP) using an anti-FLAG antibody. After collecting the interacting proteins through acidic elution, the samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among the proteins showing reduced levels in the DEL group, six were associated with intracellular trafficking, secretion, and vesicular transport. The intensity of the 6 proteins that interact with DEL or WT SIRT1 were represented on a heatmap (Figure 2A). Of these, only Syntaxin-binding protein 1 (STXBP1), a peripheral membrane protein, plays a crucial role in protein secretion.
HostingRepository	iProX
AnnounceDate	2025-03-25
AnnouncementXML	Submission_2025-03-26_01:17:17.315.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuo Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-26 01:16:52	ID requested
⏵ 1	2025-03-26 01:17:17	announced

Dataset with its publication pending

submitter keyword: SIRT1，STXBP1

Cao Liu
contact affiliation	China Medical University
contact email	lcao@cmu.edu.cn
lab head
Zhuo Wang
contact affiliation	China Medical University
contact email	wangz@cmu.edu.cn
dataset submitter

iProX dataset URI