⮝ Full datasets listing
PXD062232-1
PXD062232 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cooperative roles of class 1A PI3K isoforms in cell survival and proliferation in translocation-related sarcoma |
| Description | Among class 1 phosphatidylinositol 3-kinase (PI3K) isoforms, the isoform that predominantly regulates proliferation and survival in cancers dependent on PI3K signaling differs according to the genetic background and lineage of origin. We previously reported that translocation-related sarcomas (TRSs) including synovial sarcoma are highly susceptible to pan-class 1 PI3K inhibitors, but the dominant isoform remains unclear. In this study, we found that proliferation and survival in TRS cells were predominantly regulated by PI3Kα, with only minor contributions from PI3Kβ, PI3Kδ, and PI3Kγ. Interestingly, the combinatorial inhibition of PI3Kα, PI3Kβ, and PI3Kδ fully potentiate the antitumor activity and eventually induce apoptosis. To investigate the mechanism of the synergistic antitumor effects by the combinatorial inhibition, we performed global phosphoproteomic analysis of Aska-SS synovial sarcoma cell line treated with the PI3Kα-specific inhibitor alpelisib or the pan-PI3K inhibitor ZSTK474. We found that ZSTK474 inhibits Akt/mTOR signaling more strongly than alpelisib. Based on the results of phosphoproteomic analysis and subsequent validation experiments, we concluded that PI3Kα is the dominant isoform, while PI3Kβ and PI3Kδ can compensate for the function of PI3Kα and sustain the robustness of TRS cells in tumor proliferation and survival. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-03-25 |
| AnnouncementXML | Submission_2026-03-24_08:00:15.637.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sho Isoyama |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-25 18:43:20 | ID requested | |
| ⏵ 1 | 2026-03-24 08:00:16 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: synovial sarcoma, PI3K, phosphoproteomics |
Contact List
| Sho Isoyama | |
|---|---|
| lab head | |
| Sho Isoyama | |
| contact affiliation | Japanese Foundation for Cancer Research |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
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