PXD062219 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative mapping of methionine sensitivity to oxidation in the two-copper chaperone PcuC from Cereibacter sphaeroides |
| Description | Copper is typically coordinated by histidine, cysteine, or methionine residues in enzyme or chaperones and these residues are particularly sensitive to oxidation. However, it remains unclear whether copper-coordinating residues exhibit different sensitivity to oxidation compared to their non-coordinating counterparts, nor whether their oxidation susceptibility varies between the apo and copper-bound states of the protein. To evaluate this, we combined redox proteomics to structural analysis using the Cereibacter sphaeroides copper chaperone PcuC, which participates in the assembly of bacterial respiratory cytochrome oxidase. PcuC contains a canonical copper binding site made of two Met and two His (H51xnM63x22H86xM88), binding a Cu(I), and an unfolded C-terminal extension, rich in Met and His, proposed to coordinate an extra Cu atom. Our results showed that, in the apo-PcuC, Met135 in the C-terminal extension is highly sensitive to oxidation contrary to copper-coordinating Met, but that in the holo-PcuC, the presence of Cu strongly increased their sensitivity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-09 |
| AnnouncementXML | Submission_2026-02-08_16:22:05.518.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Maya BELGHAZI |
| SpeciesList | scientific name: Cereibacter sphaeroides; NCBI TaxID: NEWT:1063; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-25 09:35:26 | ID requested | |
| ⏵ 1 | 2026-02-08 16:22:06 | announced | |
Publication List
| Tarrago L, Molinelli L, Belghazi M, Tribout M, Lemaire D, Legrand P, Grosse S, Pignol D, Sabaty M, Tron T, Arnoux P, Quantitative mapping of methionine sensitivity to oxidation in the copper-bound PcuC chaperone. Redox Biol, 90():104037(2026) [pubmed] |
| 10.1016/j.redox.2026.104037; |
Keyword List
| submitter keyword: methionine oxidation, cofactor biosynthesis, periplasm,copper chaperone, oxaziridine |
Contact List
| Lionel Tarrago |
|---|
| contact affiliation | Fungal Biodiversity and Biotechnology Laboratory team "Responses and adaptation of fungi to environmental constraints" 163 Avenue de Luminy, CP925 13288 Marseille, Cedex 9, FRANCE |
| contact email | lionel.tarrago@inrae.fr |
| lab head | |
| Maya BELGHAZI |
|---|
| contact affiliation | CNRS |
| contact email | maya.belghazi@univ-amu.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062219
- Label: PRIDE project
- Name: Quantitative mapping of methionine sensitivity to oxidation in the two-copper chaperone PcuC from Cereibacter sphaeroides
