PXD062209-1

PXD062209 is an original dataset announced via ProteomeXchange.

Title	The composition and structure of the outer kinetochore KMN complex is conserved across Kingdoms
Description	In eukaryotes, chromosome segregation during cell division requires attachment to the spindle, which is ensured by a large multi-protein structure called the kinetochore. The outer layer of the kinetochore attaches to the microtubules and is named after three of its components KNL1C, MIS12C, and NDC80C (KMN). The KMN complex comprises ten proteins in humans and eleven in S. cerevisiae, whether it is fully conserved in more distant eukaryotes is unclear. Here, we aimed to define the KMN complex in the plant clade using co-immunoprecipitations in Arabidopsis cell cultures. We identified thirteen KMN proteins, including seven with previously known conserved functions (atMIS12, atNNF1, atNDC80, atSPC24, atSPC25, atNUF2, and atKNL1) and six that were uncharacterized. These six proteins show remote similarity to the four yeast/human KMN components whose homologs have not been characterized in plants so far. Accordingly, we named these proteins atDSN1, atCSM1, atNSL1A/B, and atZWINT1A/B. We showed that atDSN1, atCSM1, atNSL1A, and atZWINT1A localize to the kinetochore in planta and that atDSN1, atCSM1, and ZWINT1A/B are essential, further supporting their kinetochore function. AlphaFold2 predictions support an alike3D organization of the KMN complex in plants and mammals. We conclude that the KMN complex is globally conserved with an identical composition and similar organization in very distant eukaryotes, and was likely present in the common ancestors of all living eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-16_16:27:17.878.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062209
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominique Eeckhout
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-25 07:00:45	ID requested
⏵ 1	2025-11-16 16:27:18	announced

10.6019/PXD062209;

10.1038/s42003-025-09120-6;

Singh DK, Walkemeier B, Nayini A, Van Leene J, Durand S, De Jaeger G, Guerois R, Mercier R, The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms. Commun Biol, 8(1):1543(2025) [pubmed]

submitter keyword: AP-MS,Arabidopsis, kinetochore, KMN complex

Geert De Jaeger
contact affiliation	Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
contact email	geert.dejaeger@psb.vib-ugent.be
lab head
Dominique Eeckhout
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium
contact email	dominique.eeckhout@psb.ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062209
     1. Label: PRIDE project
     2. Name: The composition and structure of the outer kinetochore KMN complex is conserved across Kingdoms