PXD062208-1

PXD062208 is an original dataset announced via ProteomeXchange.

Title	Measuring the hydrodynamic radii of peptides and proteins with an unmodified LC-ESI-MS instrument operating in a Taylor dispersion regime
Description	Native ion mobility – mass spectrometry has been well established in recent years as a structural biology method. However, it is still useful to combine gas-phase techniques with complementary solution-phase measurements, for example hydrodynamic radius (Rh) determination. A common method to measure protein Rh is the use of Taylor dispersion analysis (TDA), which relies on measuring the longitudinal diffusion of an analyte in a laminar flow within a capillary. This type of analysis most often relies on spectroscopic (e.g., fluorescence-based) detection, although a few studies have reported the use of custom-built or modified fluidics systems coupled with MS-based detection. Here, we have repurposed a standard, unmodified LC-MS system, operated without a column, to measure hydrodynamic radii of peptides and proteins, including noncovalent complexes, ranging from 300 Da to 133 kDa. The Rh values we measured showed excellent correlation with gas-phase collision cross-sections as well as Rh values measured with a conventional TDA instrument with fluorescence-based detection. Notably, due to the mass-selective readout of our method, which is based on analysis of extracted ion chromatograms, we were able to perform multiplexed Rh measurements of several proteins in a single experiment, and also measured apparent Rh values for different oligomeric states of BSA and concanavalin A. Consistent with prior literature, the latter data suggested that concanavalin A primarily exists as a dimer and tetramer in solution, with monomer signals being largely due to dissociation during electrospray ionisation. We have named our method Hydrodynamic Radii from an Unmodified Liquid Chromatography - Mass Spectrometer (HYDRAULIC-MS).
HostingRepository	PRIDE
AnnounceDate	2025-07-10
AnnouncementXML	Submission_2025-07-10_00:43:26.512.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Eisert
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-25 06:53:59	ID requested
⏵ 1	2025-07-10 00:43:26	announced

10.1039/D5AN00344J;

submitter keyword: LC-MS, Hydrodynamic radius, CCS, Taylor dispersion

Prof. Frederik Lermyte
contact affiliation	Department of Chemistry, Clemens-Schöpf-Institute of Chemistry and Biochemistry, Technical University of Darmstadt, Peter-Grünberg-Strasse 4, 64287 Darmstadt, Germany
contact email	frederik.lermyte@tu-darmstadt.de
lab head
Jonathan Eisert
contact affiliation	Department of Chemistry, Clemens-Schöpf-Institute of Chemistry and Biochemistry, Technical University of Darmstadt, Peter-Grünberg-Strasse 4, 64287 Darmstadt, Germany
contact email	jonathan.eisert@tu-darmstadt.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062208
     1. Label: PRIDE project
     2. Name: Measuring the hydrodynamic radii of peptides and proteins with an unmodified LC-ESI-MS instrument operating in a Taylor dispersion regime