PXD062184-1

PXD062184 is an original dataset announced via ProteomeXchange.

Title	Probing kinase proximity interactome modulation upon drug treatment by miniTurboID proximity labelling
Description	In a systematic effort to profile kinase inhibitors, which also act as degraders, we identified multiple hit compounds. These were subjected to detailed mechanism of action elucidation including analysis of the perturbed interactome and its effect on degradation. For our first example, LYN, Src inhibitor 3 showed rapid degradation at nanomolar potencies. With BioID we could identify that two E3 ligases CBL and CBL-B are both required for this degradation mechanism, which we validated orthogonally by CRISPR/Cas9 k.o. For the second example, we identified TAK285 to degrade BLK in a γ-secretase dependent manner. We thus performed a two-pronged BioID approach using both BLK as the bait as well as APH1A, a subunit of the γ-secretase complex. We could identify an interaction with multiple components involved in γ-secretase processing for BLK, namely NCTSN and ADAM10. As a third example, we investigated the proximity interactome of RIPK2, upon inhibitor/degrader treatment.
HostingRepository	PRIDE
AnnounceDate	2025-09-04
AnnouncementXML	Submission_2025-09-04_01:10:09.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabian Frommelt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-24 19:20:32	ID requested
⏵ 1	2025-09-04 01:10:09	announced

Dataset with its publication pending

submitter keyword: kinase inhibitors,TMT

Georg E. Winter
contact affiliation	Winter Lab, CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria
contact email	gwinter@cemm.oeaw.ac.at
lab head
Fabian Frommelt
contact affiliation	CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
contact email	fabian.frommelt@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062184

PRIDE project URI

• PRIDE
  1. PXD062184
     1. Label: PRIDE project
     2. Name: Probing kinase proximity interactome modulation upon drug treatment by miniTurboID proximity labelling