PXD062183-1

PXD062183 is an original dataset announced via ProteomeXchange.

Title	Enrichment and LC-MS/MS analysis of S-acylated proteins
Description	HeLa cells were lysed and free cysteines capped with iodoacetamide. S-acylation was hydrolysed with hydroxylamine and newly revealed Cys allowed to react with an alkyne-tagged probe. Proteins were clicked with azido-biotin, enriched on neutravidin beads, digested with trypsin and desalted.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-29_05:04:57.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EMMANUELLE THINON
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-24 15:33:02	ID requested
⏵ 1	2026-06-29 05:04:57	announced

10.1021/ACSCHEMBIO.5C00824;

10.1021/acschembio.5c00824;

submitter keyword: acyl exchange,S-acylation

Emmanuelle Thinon
contact affiliation	Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB, F-33600 Pessac, France
contact email	e.thinon@iecb.u-bordeaux.fr
lab head
EMMANUELLE THINON
contact affiliation	CNRS
contact email	e.thinon@iecb.u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD062183

PRIDE project URI

• PRIDE
  1. PXD062183
     1. Label: PRIDE project
     2. Name: Enrichment and LC-MS/MS analysis of S-acylated proteins