PXD062157-1

PXD062157 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling in HeLa Cells
Description	This study combined co-immunoprecipitation (Co-IP) and LC-MS/MS proteomics in MARCH8-overexpressing HeLa cells to identify potential substrates of the E3 ubiquitin ligase MARCH8. Using empty vector-transfected cells as controls, we generated a comprehensive protein interaction dataset to elucidate MARCH8’s regulatory mechanisms in membrane protein trafficking and degradation, providing a resource for exploring its roles in viral defense, immune modulation, and related pathologies.
HostingRepository	iProX
AnnounceDate	2025-03-23
AnnouncementXML	Submission_2025-12-04_16:56:22.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liang Wei
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-23 20:10:03	ID requested
⏵ 1	2025-12-04 16:56:23	announced

Wei L, Zhao F, Liu X, Mei S, Huang Y, Xie Y, Hu Y, Wang L, Wang L, Gao Z, Chen C, Shi Y, He Y, Wang J, Xue T, Xu F, Fang J, Guo F, MARCH8-mediated ubiquitination regulates expression of the antiviral protein IFITM3. J Biol Chem, 301(12):110879(2025) [pubmed]

submitter keyword: MARCH8-Interacting Proteins

Fei Guo
contact affiliation	National Institute of Pathogen Biology, Chinese Academy of Medical Sciences
contact email	guoafei@hotmail.com
lab head
Liang Wei
contact affiliation	National Institute of Pathogen Biology, Chinese Academy of Medical Sciences
contact email	liangwei_6@163.com
dataset submitter

iProX dataset URI