PXD062138-1

PXD062138 is an original dataset announced via ProteomeXchange.

Title	Rhizosphere bacterial community in re-used cover crop root channels initiate responses against drought
Description	Plant growth and development depends on the availability of nutrients and water resources in the soil. The increased frequencies of drought events over recent years have affected nutrient availability soil systems. Crops reutilising winter cover crop root channels allows access to resources from distal regions in the soil horizon. However, the availability of information regarding root channel reutilisation under drought, specifically bacterial community structures and functions is unknown. In this study, we observed the changes inflicted by drought on bacterial communities in maize (Zea mays L.) rhizospheres after reusing the winter cover crop root channels. The techniques of 16S rRNA (ribosomal ribonucleic acid) gene-based microbial profiling and metaproteomics were used to study the alterations in biochemical pathways of those communities under drought at three different locations (Hohenschulen, Karkendamm and Reinshof) comprising three different soli types (Luvisol, Podzol and Phaeozem) respectively. Besides the influence of soil properties, we noticed that under drought the relative abundances of Acidobacteriota, Actinomycetota, Planctomycetota, and Pseudomonadota increased, while Chloroflexota, Methylomirabilota, Patescibacterota, and Verrucomicrobiota decreased. At drought-prone soil types Luvisol and Podzol, aerobic communities Pseudomonadota and Verrucomicrobiota increased abundance of the glyoxylate cycle as a means of conserving carbon and energy for plausible survival measures. Higher abundance of catalase-glutathione peroxidase (CAT-PER) through the methionine cycle-transsulfuration pathway possibly alleviate reactive oxygen species (ROS) levels. Overall, bacterial communities in the reused cover crop root channels respond to drought by taking mitigative measures for survival.
HostingRepository	PRIDE
AnnounceDate	2025-09-23
AnnouncementXML	Submission_2025-09-23_09:44:59.789.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Debjyoti Ghosh
SpeciesList	scientific name: soil metagenome; NCBI TaxID: 410658;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-21 22:08:58	ID requested
⏵ 1	2025-09-23 09:45:00	announced

10.1111/GCB.70512;

submitter keyword: Cover crop

root channel re-use

bacterial community

drought

soil types

metaproteomics

Nico Jehmlich
contact affiliation	Group Leader Microbiome Biology Department of Molecular Toxicology Helmholtz Centre for Environmental Research - UFZ GmbH, Permoserstraße 15, 04318 Leipzig, Saxony, Germany
contact email	nico.jehmlich@ufz.de
lab head
Debjyoti Ghosh
contact affiliation	Department of Molecular Toxicology, Helmholtz Centre for Environmental Research - UFZ GmbH, Permoserstraße 15, 04318 Leipzig, Saxony, Germany
contact email	debjyoti.ghosh@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062138
     1. Label: PRIDE project
     2. Name: Rhizosphere bacterial community in re-used cover crop root channels initiate responses against drought