PXD062128 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis links truncated tau to lysosome motility, autophagy and endo-lysosomal dysfunction |
| Description | Tauopathies are characterized by the progressive accumulation of abnormal tau species, which disrupt the autophagy-lysosomal pathway (ALP), a critical system for degrading intracellular macromolecules and aggregated proteins, causing toxicity and cell death. This study investigates the impact of the N-terminally truncated Tau35 protein overexpression on proteolytic pathways, including effects on autophagy and endo-lysosomal processes. Using a Tau35 mouse model and SH-SY5Y cell lines stably expressing either the Tau35 fragment or full-length tau, we employed western blotting, proteomic analysis of lysosome-enriched brain fractions, proteolysis/endocytosis assays, and live-cell imaging with the lysotracker reporter to assess protein degradation and lysosomal function. Our findings identify early pathological changes in endo-lysosomal processes, including increased endocytosis, proteolytic dysfunction and lysosomal motility abnormalities, associated with Tau35- induced toxicity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-22 |
| AnnouncementXML | Submission_2025-12-21_17:04:54.488.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD062128 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Steven Lynham |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-21 10:41:30 | ID requested | |
| ⏵ 1 | 2025-12-21 17:04:55 | announced | |
Publication List
| Goniotaki D, Hausherr M, Lynham S, Ale A, Chennell G, Marcotti S, Marcus K, Noble W, Hanger DP, Fraser G, Srivastava DP, Proteomic analysis links truncated tau to lysosome motility, autophagy, and endo-lysosomal dysfunction. Alzheimers Dement, 21(12):e70977(2025) [pubmed] |
| 10.1002/alz.70977; |
| 10.6019/PXD062128; |
Keyword List
| submitter keyword: Tauopathies, toxicity, proteomics, mass spectrometry, autosomal |
Contact List
| Prof. Deepak Srivastava |
|---|
| contact affiliation | King's College London, Dept. of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience Institute, 5 Cutcombe Road, London, SE5 9NU |
| contact email | deepak.srivastava@kcl.ac.uk |
| lab head | |
| Steven Lynham |
|---|
| contact affiliation | King's College London |
| contact email | steven.lynham@kcl.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD062128 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062128
- Label: PRIDE project
- Name: Proteomic analysis links truncated tau to lysosome motility, autophagy and endo-lysosomal dysfunction
