PXD062126-1

PXD062126 is an original dataset announced via ProteomeXchange.

Title	An Essential Factor for Apicoplast Fission and Inheritance in Malaria Parasites
Description	Blood-stage Plasmodium falciparum parasites rely on a non-photosynthetic plastid, the apicoplast, for survival, making it an attractive target for antimalarial intervention. Like the mitochondrion, the apicoplast cannot be generated de novo and must be inherited by daughter parasites during cell division. This process relies on coordinated fission events, yet the molecular mechanisms remain poorly understood. Here, we identify a previously uncharacterized P. falciparum protein (Pf3D7_0613600), which we name PfAnchor, as a key regulator of apicoplast fission. Using Ultrastructure Expansion Microscopy (U-ExM), we show that PfAnchor localizes to the apicoplast throughout the asexual life cycle. Conditional depletion disrupts apicoplast fission, leading to incomplete cytokinesis and parasite death. Notably, loss of apicoplast branching via azithromycin treatment rescues these defects, underscoring Anchor’s specific role in apicoplast division. Immunoprecipitation further identifies an interaction with the dynamin-like GTPase PfDyn2, a key mediator of both apicoplast and mitochondrial fission, establishing PfAnchor as the first apicoplast-specific dynamin adaptor protein. Our findings define PfAnchor as an essential factor for apicoplast fission and inheritance in P. falciparum blood-stage parasites, highlighting parasite-specific organelle division as a potential vulnerability for therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2025-10-27
AnnouncementXML	Submission_2025-10-27_14:29:10.938.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062126
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: NEWT:36329;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-21 09:09:53	ID requested
⏵ 1	2025-10-27 14:29:11	announced

Blauwkamp J, Rajaram K, Staggers SR, Harrigan O, Doud EH, Prigge ST, Sun SY, Absalon S, An Essential Adaptor for Apicoplast Fission and Inheritance in Malaria Parasites. Res Sq, ():(2025) [pubmed]

10.21203/rs.3.rs-6457426/v1;

10.6019/PXD062126;

submitter keyword: Plasmodium falciparum, mitochondrial fission, PfAnchor, apicoplast, GTPase, dynamin adaptor protein

Amber Mosley
contact affiliation	Indiana University School of Medicine, USA
contact email	almosley@iu.edu
lab head
Emma Doud
contact affiliation	Indiana University School of Medicine
contact email	edoud@iu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062126
     1. Label: PRIDE project
     2. Name: An Essential Factor for Apicoplast Fission and Inheritance in Malaria Parasites