PXD062104-1

PXD062104 is an original dataset announced via ProteomeXchange.

Title	Mouse Liver Kupffer Cells LC-MS MS
Description	The importance of immunometabolism in the development of metabolic diseases is clear. Yet, how certain metabolic disorders, such as insulin deficiency (ID), influence immune cell function, and vice versa, is poorly understood. Also, therapeutic strategies to harness the interplay between immune cells and metabolism are lacking. Here, we observe that ID rearranges the immune landscape of the liver, causing a decrease of T cells and an increase of the Kupffer cells, accompanied by a shift in the transcriptional signature and polarization of the latter. Treating ID mice with the protein S100A9 rescues the polarization and lipid-related changes caused by ID in the KCs, and, through them, rescues hypertriglyceridemia and hyperketonemia in a TLR4-dependent manner. Additionally, S100A9 acts on other immune niches to increase glucose uptake in the skeletal muscle, improving hyperglycemia. In summary, our findings pinpoint the S100A9-TLR4 axis as a new tool to harness immune cells for improving ID-related metabolic dysfunction.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:44:03.138.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giulia Lucibello
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-21 02:30:58	ID requested
⏵ 1	2025-09-14 16:44:04	announced

Lucibello G, Ursino G, Teixeira PDS, Zahoran S, Fanuele F, Kallikourdis M, Visentin F, Veyrat-Durebex C, Widmer A, Wu Y, Cremonesi M, Wollheim CB, Castets P, Ramadori G, Coppari R, Harnessing Distinct Tissue-Resident Immune Niches via S100A9/TLR4 Improves Ketone, Lipid, and Glucose Metabolism. Endocrinology, 166(10):(2025) [pubmed]

10.1210/endocr/bqaf131;

submitter keyword: Mouse, Kupffer cells, Liver, diabetes

Roberto Coppari
contact affiliation	University of Geneva, Faculty of Medicine, Department of Cell physiology and Metabolism
contact email	roberto.coppari@unige.ch
lab head
Giulia Lucibello
contact affiliation	University of Geneva
contact email	giulia.lucibello@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062104

PRIDE project URI

• PRIDE
  1. PXD062104
     1. Label: PRIDE project
     2. Name: Mouse Liver Kupffer Cells LC-MS MS