PXD062091-1

PXD062091 is an original dataset announced via ProteomeXchange.

Title	Nascent proteomic profiling of a METTL13-dependent oncogenic translational program
Description	We previously identified an orphan protein METTL13 (methyltransferase-like protein13) as the physiologic enzyme that specifically catalyzes dimethylation of eEF1A at lysine 55 (eEF1AK55me2). Targeting of METTL13 may have potent anti-cancer activity while causing minimal toxicity in normal tissues. The mechanism whereby the METTL13-eEF1AK55me2 alters mRNA translation to drive tumorigenesis is obscure. We have previously shown that eEF1AK55me2 bolsters bulk protein synthesis by promoting global elongation rates. Nonetheless, the effects of eEF1AK55me2 on selective reprogramming of protein synthesis are still unknown. Importantly selective reprograming of the translatome is required to establish malignant proteomes that fuel neoplastic growth.To directly investigate the role of eEF1AK55me2 in the establishment of malignant proteomes, a quantitative noncanonical amino acid tagging (QuaNCAT) strategy was used for global profiling of protein synthesis in cultured cell lines, focusing our analyses on T3M4 cells, a KRASQ61H-harboring pancreatic cancer cell line in which translation rates are known to be regulated by METTL13.
HostingRepository	PRIDE
AnnounceDate	2026-08-15
AnnouncementXML	Submission_2026-08-14_18:53:54.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuo Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-20 16:14:05	ID requested
⏵ 1	2026-08-14 18:53:54	announced

Dataset with its publication pending

submitter keyword: SILAC

METTL13

eEF1AK55 dimethylation

newly synthesized protein

cancer

Shuo Liu
contact affiliation	Stanford University
contact email	sliu12@stanford.edu
lab head
Shuo Liu
contact affiliation	Stanford University
contact email	sliu12@stanford.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062091
     1. Label: PRIDE project
     2. Name: Nascent proteomic profiling of a METTL13-dependent oncogenic translational program