PXD062079-1

PXD062079 is an original dataset announced via ProteomeXchange.

Title	Analysis of the nuclear proteome fraction of rAAV2-plasmid transfected HEK-293 cells during AAV production
Description	In this comparative proteomic analysis, we focused on proteins in the nuclear fraction. As the nucleus is the epicenter of DNA uptake, transcription, capsid assembly and packaging. Three HEK-293 cell samples i) not transfected, ii) MOCK plasmid transfected, and iii) triple-transfected with rAAV2-production plasmids were harvested 24 h and 72 h after transfection. In total 3384 proteins in all samples were identified revealing regulated proteins due to transfection and rAAV production.
HostingRepository	PRIDE
AnnounceDate	2025-07-21
AnnouncementXML	Submission_2025-07-20_16:55:15.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raimund Hoffrogge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-20 07:18:20	ID requested
⏵ 1	2025-07-20 16:55:16	announced

10.3390/ijms26136315;

Golm SK, Hoffrogge R, M, ü, ller KM, Nuclear Fraction Proteome Analyses During rAAV Production of AAV2-Plasmid-Transfected HEK-293 Cells. Int J Mol Sci, 26(13):(2025) [pubmed]

submitter keyword: MS/MS, nuclear fraction, FASP,rAAV production

Prof. Kristian Marc Müller
contact affiliation	Cellular and Molecular Biotechnology, Faculty of Technology, Bielefeld University, Germany
contact email	kristian@syntbio.net
lab head
Raimund Hoffrogge
contact affiliation	Bielefeld University, Cell Culture Technology
contact email	raimund.hoffrogge@uni-bielefeld.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062079
     1. Label: PRIDE project
     2. Name: Analysis of the nuclear proteome fraction of rAAV2-plasmid transfected HEK-293 cells during AAV production