PXD062075-1

PXD062075 is an original dataset announced via ProteomeXchange.

Title	Cytotoxicity and effector cooperation in the type III secretion system of Aeromonas schubertii
Description	The type III secretion system (T3SS) is an important virulence factor of Gram-negative bacteria, including the genus Aeromonas, a group of aquatic bacteria capable of both mutualistic and pathogenic interactions. Aeromonas species are increasingly recognized as opportunistic human pathogens. The type strain A. schubertii ATCC 43700 encodes two distinct T3SSs located in the Aeromonas pathogenicity islands 1 and 2, hereby designated as API1 and API2, respectively. This work investigates the role of API1 and API2 in A. schubertii-induced cytotoxicity and identifies novel type III secretion effectors. HeLa cell infections showed that API1, but not API2, is essential for cellular cytotoxicity resulting in both apoptotic and necrotic cell death. The ΔAPI1 mutant failed to induce cytotoxicity, whereas the wild-type (WT) and ΔAPI2 strains induced comparable cytotoxic effects. Proteomic analysis identified 7 candidate effectors secreted by the API1 injectisome under low-calcium conditions. These included two previously characterized effectors, AopH and AopO of A. salmonicida, and five novel effectors hereby named AopI, AopJ, AopL, AopT, and AopU, whose injection into host cells via API1 was validated using a split luciferase reporter system. Functional analysis revealed distinct roles for these effectors. AopL, homologous to the VopQ effector of Vibrio cholerae, accelerated caspase 3-independent necrosis, while AopI, homologous to ExoY of Pseudomonas aeruginosa, suppressed caspase activation and necrosis, indicating a pro-survival function. These results show the role of API1 injectisome in the cytotoxicity of A. schubertii and expand our understanding of T3SS-mediated host-pathogen interactions in Aeromonas species.
HostingRepository	PRIDE
AnnounceDate	2025-06-10
AnnouncementXML	Submission_2025-06-10_00:55:55.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Jurnečka
SpeciesList	scientific name: Aeromonas schubertii; NCBI TaxID: 652;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-20 06:36:55	ID requested
⏵ 1	2025-06-10 00:55:56	announced

10.1186/S13567-025-01548-2;

submitter keyword: Type III secretion system,Aeromonas schubertii

Jana Kamanova
contact affiliation	Laboratory of Infection Biology Institute of Microbiology, v.v.i. The Czech Academy of Sciences
contact email	kamanova@biomed.cas.cz
lab head
David Jurnečka
contact affiliation	Institute of Microbiology, Czech Academy of Sciences
contact email	david.jurnecka@biomed.cas.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062075
     1. Label: PRIDE project
     2. Name: Cytotoxicity and effector cooperation in the type III secretion system of Aeromonas schubertii