PXD062042 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RECQL5 and transcription-coupled DNA repair complex coordinate to regulate transcription elongation |
| Description | The RECQL5 helicase is crucial for maintaining genome stability and functions at the intersection of transcription, replication, and DNA repair. RECQL5 associates with RNA15 polymerase II (Pol II) to modulate transcription elongation, yet the underlying mechanism remains unclear. Here, we report cryo-electron microscopy structures of human RECQL5 bound to multiple transcription elongation complexes. Combined with biochemical analysis, we identified an a-helix of RECQL5 responsible for Pol II binding and inhibition of transcription elongation. We further reveal that the transcription-coupled DNA repair (TCR) complex20 reactivates the transcription of RECQL5-inhibited Pol II through concerted actions of its translocase activity and competition with the RECQL5 helix for engaging Pol II. Our results suggest a model in which RECQL5 and TCR coordinately regulate the transcription elongation rate to ensure transcription efficiency while maintaining genome stability. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-09-22 |
| AnnouncementXML | Submission_2025-09-21_16:14:09.791.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tomos Morgan |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-19 11:45:04 | ID requested | |
| ⏵ 1 | 2025-09-21 16:14:10 | announced | |
Publication List
| 10.1038/s41594-025-01586-6; |
| Zhang L, Gordiyenko Y, Morgan T, Franco C, Tufegd, ž, i, ć, Vidakovi, ć A, Zhang S, Structural basis of RECQL5-induced RNA polymerase II transcription braking and subsequent reactivation. Nat Struct Mol Biol, 32(9):1731-1740(2025) [pubmed] |
Keyword List
| submitter keyword: RECQL5, Transcription, crosslinking |
Contact List
| Suyang Zhang |
|---|
| contact affiliation | Laboratory of Molecular Biology. |
| contact email | szhang@mrc-lmb.cam.ac.uk |
| lab head | |
| Tomos Morgan |
|---|
| contact affiliation | MRC-LMB |
| contact email | tmorgan@mrc-lmb.cam.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062042 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062042
- Label: PRIDE project
- Name: RECQL5 and transcription-coupled DNA repair complex coordinate to regulate transcription elongation
