PXD062037 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Role of FN Isoforms in Myogenic Progenitors |
| Description | While Fibronectin (FN) is abundant and has pleiotropic functions during skeletal muscle regeneration, it remains unclear how its spatiotemporal specificity for skeletal muscle stem cells (MuSCs) is established. We discovered that activated MuSCs secrete an autoregulatory FN splice variant containing the EDB extra domain (EDB(+) FN), which is not expressed by accessory cells in the niche. In order to study the consequences of reduced levels of FN isoforms in MuSC-derived primary mouse myoblasts, we performed a proteomic analysis comparing cells treated with siRNAs targeting either (I) EDB(+) FN, (II) EDB(-) FN (targeting all FN transcripts without the EDB extra domain), or (III) siSCR (scrambled siRNA). |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-10-29 |
| AnnouncementXML | Submission_2025-10-29_14:22:42.981.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuguo Liu |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-19 09:26:34 | ID requested | |
| ⏵ 1 | 2025-10-29 14:22:43 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Alternative splicing, Aging, Stem cell niche, Fibronectin, Integrin,MuSCs, Smad3, Extracellular matrix, TGF, Satellite cells, Srsf1 |
Contact List
| Florian Bentzinger |
|---|
| contact affiliation | University of Sherbrooke |
| contact email | cf.bentzinger@usherbrooke.ca |
| lab head | |
| Yuguo Liu |
|---|
| contact affiliation | University of Sherbrooke |
| contact email | yuguo.liu@usherbrooke.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD062037 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062037
- Label: PRIDE project
- Name: Role of FN Isoforms in Myogenic Progenitors
