PXD062029-1

PXD062029 is an original dataset announced via ProteomeXchange.

Title	What’s left from the brew? Investigating residual barley proteins in spent grains for downstream valorization opportunities
Description	Brewer’s spent grain (BSG) is the major side-stream from beer production but remains highly underutilized. While the direct use of BSG as a food ingredient is limited due to subpar techno-functionality, the vast amounts and fairly high protein content of up to 30% makes it a high potential source for production of protein-based ingredient by valorization through e.g. enzymatic hydrolysis. However, little attention has been put towards the protein-level composition of BSG, which is essential for developing hydrolysis strategies for improving functionality in a targeted manner. Here, we present an in-depth characterization of the BSG proteome and investigate dynamic proteome changes from malting and mashing in the initial phases of beer production. We show dynamic and selective changes in the proteome across the different process steps, where 29% of reproducibly identified proteins display differential abundance. BSG represents a significantly higher proportion of intracellular protein compared to both barley and malt and has a nutritionally favorable amino acid composition. The major constituent of the BSG proteome is B3-Hordein, constituting more than 30% of the BSG protein. Moreover, we find that a large proportion (> 45%) of the BSG protein is associated with potential food safety concerns, being classified as potential allergens and antinutritional factors. Our analysis emphasizes the need for downstream processing of BSG to produce safe and functional food ingredients, while also providing protein-level insights for development of targeted hydrolysis strategies to achieve this.
HostingRepository	PRIDE
AnnounceDate	2025-10-22
AnnouncementXML	Submission_2025-10-22_05:15:44.682.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD062029
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Simon Gregersen Echers
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: NEWT:4513;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-19 06:43:19	ID requested
⏵ 1	2025-10-22 05:15:45	announced

10.1016/J.IFSET.2025.104277;

10.6019/PXD062029;

submitter keyword: enrichment analysis,Barley, BUP, BSG, Malt, var. Planet

Simon Gregersen Echers
contact affiliation	Department of Chemistry & Bioscience Aalborg University Denmark
contact email	sgr@bio.aau.dk
lab head
Simon Gregersen Echers
contact affiliation	Department of Chemistry & Bioscience Aalborg University
contact email	sgr@bio.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062029
     1. Label: PRIDE project
     2. Name: What’s left from the brew? Investigating residual barley proteins in spent grains for downstream valorization opportunities