PXD062007-1

PXD062007 is an original dataset announced via ProteomeXchange.

Title	TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi selective autophagy
Description	Golgi degradation by selective autophagy (Golgiphagy) requires receptors to direct Golgi fragments into phagophores for sequestration within autophagosomes, followed by lysosomal degradation. Here, we show that the Golgi transmembrane protein TM9SF3 is a receptor essential for Golgiphagy under nutrient stress and multiple Golgi stress conditions. TM9SF3 binds all six mammalian ATG8 proteins through its N-terminal LC3-interacting regions. TM9SF3 knockout blocks nutrient stress-induced Golgi fragmentation and reduces the targeting of Golgi fragments to autophagosomes, resulting in decreased Golgi protein degradation. Beyond nutrient stress, TM9SF3 is required for Golgiphagy induced by monensin and brefeldin A treatments, and disruptions in intra-Golgi protein glycosylation. Knockout of TM9SF3 and mutations in its LIRs both compromise protein glycosylation, whereas TM9SF3 overexpression promotes degradation of incompletely glycosylated proteins. Further, we show that TM9SF3 is required for breast cancer cell proliferation, and high TM9SF3 levels are associated with poor prognosis, implicating its function in breast cancer pathology.
HostingRepository	iProX
AnnounceDate	2025-03-18
AnnouncementXML	Submission_2025-03-18_20:14:48.534.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiejie Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-18 20:14:28	ID requested
⏵ 1	2025-03-18 20:14:48	announced

Dataset with its publication pending

submitter keyword: TM9SF3, Golgiphagy, Receptor, ATG8, Golgi degradation, Protein glycosylation, Breast cancer

Yixian Cui
contact affiliation	Wuhan University
contact email	yixian.cui@whu.edu.cn
lab head
Jiejie Yang
contact affiliation	Wuhan University
contact email	jiejyang@whu.edu.cn
dataset submitter

iProX dataset URI