PXD061990 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of methylglyoxal reactive proteins with photocaged glycating agents |
| Description | Abstract Methylglyoxal is a highly reactive metabolite that is formed spontaneously in the glycolytic pathway. The side chains of various amino acid residues react with methylglyoxal to form advanced-glycation end products (AGEs). This enzyme-independent process introduces post-translational modifications (PTMs) onto the proteins and it was long thought that the resulting AGEs primarily inhibited proteins. More recent studies have shown that these AGEs can act in signaling and feedback loops. Moreover, it was shown that a large number of proteins react reversibly with methylglyoxal. These findings led to a renewed interest in methylglyoxal induced AGEs and led to the development of novel tools and methodologies that can be used to identify the modified proteins. Many of studies are nowadays still performed by adding methylglyoxal exogenously, often in a high concentration, despite the high reactivity of methylglyoxal. We here report new photocaged-methylglyoxal derivatives that allow the direct release of methylglyoxal in the sample of interest at the desired time and experimental condition. We demonstrate that the methylglyoxal derivatives can be released in situ by irradiating the photocaged probe with UV light. Furthermore, we show that this approach is more efficient. A far larger number of proteins are labeled with the photocaged probes than with the chemically activated probes. The here reported approach should allow studying in situ glycation under physiological more relevant conditions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-17 |
| AnnouncementXML | Submission_2025-11-16_16:37:04.518.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD061990 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Horvatovich Péter |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-18 10:48:42 | ID requested | |
| ⏵ 1 | 2025-11-16 16:37:05 | announced | |
Publication List
| 10.6019/PXD061990; |
| Sokoliova S, Sardaru IR, Wargu, ł, a FP, Hermans JH, Permentier HP, Horvatovich PL, Witte MD, Identification of Methylglyoxal Reactive Proteins with Photocaged Glycating Agents. Chembiochem, 26(21):e202500275(2025) [pubmed] |
| 10.1002/cbic.202500275; |
Keyword List
| submitter keyword: proteomics, E coli, A549 cell line, photocleavable reagent,advanced-glycation end products |
Contact List
| Peter Horvatovich |
|---|
| contact affiliation | University of Groningen Faculty of Sciences and Engineering Groningen Research Institute of Pharmacy Analytical Biochemistry |
| contact email | p.l.horvatovich@rug.nl |
| lab head | |
| Horvatovich Péter |
|---|
| contact affiliation | Faculty of Science and Engineering, Groningen Research Institute of Pharmacy, Analytical Biochemistry, University of Groningen, 9713 AV Groningen, The Netherlands |
| contact email | p.l.horvatovich@rug.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061990
- Label: PRIDE project
- Name: Identification of methylglyoxal reactive proteins with photocaged glycating agents
