PXD061973 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Anti-DnaA antibodies specificity validation by mass spectrometry |
| Description | The ubiquitous protein DnaA is a chromosomal DNA replication initiator and transcription factor. It binds to 9-bp DNA sites called "DnaA-boxes" that are localized within the replication origin (oriC) and throughout chromosomal DNA, usually in the proximity of the promoter regions. During stress, bacteria undergo metabolic shifts that halt their growth and activate survival mechanisms. One outcome of these shifts is the accumulation of long chains of polyphosphate (polyP), an evolutionarily conserved linear polymer composed of up to 1,000 phosphate groups. It was previously identified that DnaA and the Lon protease bind to polyP, which stimulates Lon for the polyP-dependent DnaA proteolysis. We assumed that it have an impact not only on DnaA intracellular concentration but might also affect DNA binding pattern. To investigate this hypothesis and to analyze the DNA interaction profile of DnaA molecules remaining in stressed cells, we performed Chromatin Immunoprecipitation coupled with DNA sequencing (ChIP-seq). To exclude the presence of nonspecifically bound proteins in the sample after immunoprecipitation using anti-DnaA antibodies, mass spectrometry analysis was performed. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-02 |
| AnnouncementXML | Submission_2026-03-01_16:07:46.878.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Natalia Musiał |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-18 06:16:24 | ID requested | |
| ⏵ 1 | 2026-03-01 16:07:48 | announced | |
Publication List
| 10.1093/nar/gkag144; |
| Boguszewska E, Hirsz Z, Sroka M, Bury K, Chmura W, Strza, ł, ka A, Ko, ł, odziej M, Zakrzewska-Czerwi, ń, ska J, Konieczny I, PolyP inhibits CobB deacetylase triggering a regulatory coupling to prevent DNA replication initiation. Nucleic Acids Res, 54(4):(2026) [pubmed] |
Keyword List
| submitter keyword: sample specificity, Escherichia coli,ChIP-seq, DnaA, rabbit antibodies |
Contact List
| prof. dr hab. Igor Konieczny |
|---|
| contact affiliation | Laboratory of Molecular Biology, Intercollegiate Faculty of Biotechnology UG&MUG |
| contact email | igor.konieczny@ug.edu.pl |
| lab head | |
| Natalia Musiał |
|---|
| contact affiliation | Intercollegiate Faculty of Biotechnology UG&MUG |
| contact email | natalia.musial@phdstud.ug.edu.pl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD061973 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061973
- Label: PRIDE project
- Name: Anti-DnaA antibodies specificity validation by mass spectrometry
