PXD061971-1

PXD061971 is an original dataset announced via ProteomeXchange.

Title	Development of Motif-Specific Monoclonal Antibodies for Global Protein Citrullination Detection with Minimal Cross-Reactivity to Homocitrullination
Description	This project focuses on developing highly specific monoclonal antibodies for detecting citrullinated proteins while minimizing cross-reactivity with homocitrullination. Citrullination, a post-translational modification catalyzed by PAD enzymes, plays key roles in immunity, inflammation, and disease. However, current detection methods lack sensitivity and specificity, limiting their utility. To address this, we employed a motif-based approach using PAD sequence preferences to generate monoclonal antibodies. Rats were immunized with a pool of over 490,000 citrullinated peptides representative of human tissue proteomes, and two antibody clones could be established and validated using ELISA. For further testing, citrullinated and homocitrullinated samples were generated in vitro, and the presence and content of the respective modification confirmed using mass spectrometry. These samples, as well as ionomycin-activated human neutrophils, were used to evaluate antibody performance in western blotting, where they demonstrated high sensitivity, specificity, and quantitative detection capabilities. These novel antibodies provide valuable tools for studying citrullination dynamics and have promising applications in biomarker discovery and disease diagnostics.
HostingRepository	PRIDE
AnnounceDate	2026-03-29
AnnouncementXML	Submission_2026-03-29_11:03:53.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophia Laposchan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carbamoylated residue; L-citrulline
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-18 05:35:33	ID requested
⏵ 1	2026-03-29 11:03:53	announced

10.1016/J.CRMETH.2026.101345;

submitter keyword: None

Chien-Yun Lee
contact affiliation	Young Investigator Group: Mass Spectrometry in Systems Neurosciences, School of Life Sciences, Technical University of Munich, Freising, Germany
contact email	chienyun.lee@tum.de
lab head
Sophia Laposchan
contact affiliation	Young Investigator Group: Mass Spectrometry in Systems Neurosciences, Technical University of Munich
contact email	sophia.laposchan@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD061971

PRIDE project URI

• PRIDE
  1. PXD061971
     1. Label: PRIDE project
     2. Name: Development of Motif-Specific Monoclonal Antibodies for Global Protein Citrullination Detection with Minimal Cross-Reactivity to Homocitrullination