PXD061942-1

PXD061942 is an original dataset announced via ProteomeXchange.

Title	Untargeted LC-MS/MS proteomics on supernatant of endothelial cells exposed to Myeloperoxidase-oxidized LDLs (Mox-LDLs)
Description	Myeloperoxidase-oxidized LDLs (Mox-LDLs) trigger endothelial cells and participate in atherosclerosis. However, the mechanisms behind Mox-LDLs stimulation are not fully understood. Therefore, we used an untargeted proteomics approach to analyze human umbilical vein endothelial cells (HUVECs) after stimulation by Mox-LDLs. HUVECs (n=6) were exposed for 24 h to Mox-LDLs (0 or 100 µg/ml) with or without native LDLs (0 or 1 mg/m). Supernatant and cell lysate were then analyzed using LC-MS/MS. Mox-LDLs treatment of HUVECs led to changes in the expression of proteins implicated in hemostasis, cell adhesion, angiogenesis, inflammation and stress responses. We also observed cell reprogramming through increased mitochondrial activity. Mox-LDLs seem to induce mitochondrial activation and oxidative stress in the cell, likely reactive oxygen species mediated. We suggest that HUVECs then activate cytoprotective antioxidant coping mechanisms (glutathione synthesis, heme oxygenase-1) to survive. Mox-LDLs may also modulate hemostasis and inflammatory responses, both pro- and anti-inflammatory.
HostingRepository	PRIDE
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2025-10-09_03:22:35.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xavier Bisteau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-17 16:57:58	ID requested
⏵ 1	2025-10-09 03:22:35	announced

10.1016/J.BBAGEN.2025.130865;

submitter keyword: supernatant,Endothelial cell, DIA, cell lysate, LC-MS/MS

Pierre Van Antwerpen
contact affiliation	ULB - Faculty of PHARMACY RD3 - Pharmacognosy, Bioanalysis & Drug Discovery Unit & Analytical Platform of the Faculty of Pharmacy
contact email	pierre.van.antwerpen@ulb.be
lab head
Xavier Bisteau
contact affiliation	IRIBHM J.E Dumont, Faculty of Medicine, Université Libre de Bruxelles
contact email	xavier.bisteau@ulb.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061942
     1. Label: PRIDE project
     2. Name: Untargeted LC-MS/MS proteomics on supernatant of endothelial cells exposed to Myeloperoxidase-oxidized LDLs (Mox-LDLs)