PXD061915-1

PXD061915 is an original dataset announced via ProteomeXchange.

Title	Proteomics Reveals Increased Periostin in Synovial Fluid from Canine and Human Anterior Cruciate Ligament Injury
Description	Post-traumatic osteoarthritis (PTOA) is a common sequela to anterior cruciate ligament (ACL) injury in humans and dogs. Identification of conserved targets between species may hasten biomarker identification and therapeutic development. To identify differentially regulated synovial fluid (SF) proteins in ACL injury, SF samples were collected from knees with ACL injury or control knees (n=8 per species) in human and dog patients. The SF proteome was evaluated using nano-scale reverse phase chromatography and tandem mass spectroscopy. Associations between proteins and ACL injury were analyzed in both species. Immunoassays were used to validate key proteins identified via mass spectrometry, including periostin, α-2-macroglublin, and lubricin, in additional SF samples (dog: n=57 ACL, n=31 controls; human: n=20 ACL, n=15 advanced OA) and plasma samples (human: n=18 ACL, n=15 advanced OA, and n=12 controls). Periostin was the most upregulated SF protein (log2 fold-change) in both dog (2.6) and human (3.5) ACL injury, followed by complement C1q (2.3) and α-2-macroglobulin (1.8) in dogs and fibrinogen (1.1) and α-2-macroglobulin (1.1) in humans. Top downregulated proteins included serum amyloid A1 (-1.9) and aggrecan (-1.8) in dogs and carbonic anhydrase 2 (-3.2) and hemoglobin subunit β (-2.8) in humans. Approximately 60% of proteins detected were shared between species, and immunoregulatory and macromolecular transport proteins were the most common families. Clinical Relevance: Findings in both species support further investigation into periostin as a potential biomarker or therapeutic target and the use of spontaneous ACL injury in dogs as a potential translational large animal model for human ACL injury.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:15:03.192.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sydney Womack
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-17 03:46:59	ID requested
⏵ 1	2025-06-15 16:15:04	announced

10.1002/jor.26078;

Womack SJ, Carballo CB, Secor EJ, Rodeo SA, Reesink HL, Proteomics Reveals Increased Periostin in Synovial Fluid From Canine and Human Anterior Cruciate Ligament Injury. J Orthop Res, 43(7):1239-1249(2025) [pubmed]

submitter keyword: canine, alpha-2-macroglobulin, proteomics, osteoarthritis, periostin, human,ACL, LC-MS/MS

Heidi Lynn Reesink
contact affiliation	Department of Clinical Sciences, Cornell University, Ithaca, NY, USA Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA
contact email	hlr42@cornell.edu
lab head
Sydney Womack
contact affiliation	Cornell University
contact email	sjw262@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD061915

PRIDE project URI

• PRIDE
  1. PXD061915
     1. Label: PRIDE project
     2. Name: Proteomics Reveals Increased Periostin in Synovial Fluid from Canine and Human Anterior Cruciate Ligament Injury